Tayoub Ghaleb, Al-Odat Mohmad, Amer Amal, Aljapawe Abdulmunim, Ekhtiar Adnan
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria.
Department of Radiation Protection and Safety, Atomic Energy Commission of Syria, Damascus, Syria.
Iran J Med Sci. 2018 Jan;43(1):52-64.
Plants are an important natural source of compounds used in cancer therapy. contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro.
Leaves, flowers, roots, and bulbs of were collected and their contents were extracted and diluted to different concentrations that were applied on MDA-MB-231 cells and normal human lymphocytes cell in vitro for different intervals. Cells viability, proliferation, cell cycle distribution, apoptosis, and growth were evaluated by flow cytometry and microscopy. Parametric unpaired t-test was used to compare effects of plant extracts on treated cell cultures with untreated control cell cultures. IC was also calculated.
extract had profound effects on MDA-MB-321 cells. It inhibited cell proliferation in a dose- and time-dependent manner. The IC values were 0.039, 0.035, and 0.026 mg/ml after 48, 72, and 96 hours of treatment with 0.1 mg/ml concentration of bulb extract, respectively. Those values were 0.051 and 0.03 mg/ml after 72 and 96 hours for root extract, respectively, and 0.048 mg/ml after 96 hours for flower extract. There were no significant effects of bulb extracts on normal lymphocytes proliferation.
extract has anti-proliferative effects on MDA-MB-231 cell line in vitro. The effects imply the involvement of mechanisms that inhibits cell growth and arresting cells at S and G/M phases. Cyclin B1, Bcl-2, and Ki67 expression was also affected.
植物是癌症治疗中所用化合物的重要天然来源。[植物名称]含有生物碱等潜在抗癌剂。在本研究中，我们调查了[植物名称]提取物对MDA - MB - 231人上皮腺癌细胞系和体外正常淋巴细胞的抗增殖作用。
采集[植物名称]的叶、花、根和鳞茎，提取其成分并稀释至不同浓度，在体外不同时间段作用于MDA - MB - 231细胞和正常人淋巴细胞。通过流式细胞术和显微镜评估细胞活力、增殖、细胞周期分布、凋亡和生长情况。使用参数非配对t检验比较植物提取物对处理后的细胞培养物与未处理的对照细胞培养物的作用。还计算了半数抑制浓度（IC）。
[植物名称]提取物对MDA - MB - 321细胞有显著影响。它以剂量和时间依赖性方式抑制细胞增殖。用0.1 mg/ml浓度的鳞茎提取物处理48、72和96小时后，IC值分别为0.039、0.035和0.026 mg/ml。根提取物在72和96小时后的IC值分别为0.051和0.03 mg/ml，花提取物在96小时后的IC值为0.048 mg/ml。[植物名称]鳞茎提取物对正常淋巴细胞增殖无显著影响。
[植物名称]提取物在体外对MDA - MB - 231细胞系有抗增殖作用。这些作用意味着涉及抑制细胞生长并使细胞停滞在S期和G/M期的机制。细胞周期蛋白B1、Bcl - 2和Ki67的表达也受到影响。
