Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Planta Med. 2013 Oct;79(15):1480-4. doi: 10.1055/s-0033-1350741. Epub 2013 Aug 22.
As a part of ongoing search efforts for the discovery of anticancer lead entities from natural sources, bulbs and flowers of the amaryllidaceous plant Pancratium maritimum have been investigated. Fractionation of the extracts of the fresh flowers and bulbs of P. maritimum led to the isolation of four new alkaloids, namely pancrimatines A (1) and B (2), norismine (3), and pancrimatine C (4), together with the previously reported N-methyl-8,9-methylenedioxy-6-phenanthridone (5), trispheridine (6), and N-methyl-8,9-methylenedioxyphenanthridine (7). The structures of these alkaloids were established on the basis of extensive 1D and 2D NMR and high-resolution mass spectral analyses as well as comparison with the literature. Compounds 2 and 7 showed antiproliferative and antimigratory activity against the highly metastatic human prostate cancer cell line PC-3 cells without cytotoxicity. The phenanthridine alkaloid class was identified as having potential for use to control prostate cancer proliferation and migration.
作为从天然来源中发现抗癌先导化合物的持续研究工作的一部分，对石蒜科植物 P. maritimum 的鳞茎和花朵进行了研究。对 P. maritimum 的新鲜花朵和鳞茎提取物进行分离，得到了四个新的生物碱，即 pancrimatines A（1）和 B（2）、norismine（3）和 pancrimatine C（4），以及先前报道的 N-甲基-8,9-亚甲二氧基-6-菲啶酮（5）、trispheridine（6）和 N-甲基-8,9-亚甲二氧基菲啶（7）。这些生物碱的结构是基于广泛的 1D 和 2D NMR 和高分辨率质谱分析以及与文献的比较来确定的。化合物 2 和 7 对高度转移性的人前列腺癌细胞系 PC-3 细胞表现出抗增殖和抗迁移活性，而没有细胞毒性。菲啶生物碱类被认为具有控制前列腺癌增殖和迁移的潜力。
