Qi Yongbin, Wang Linyou, Gui Junmei, Zhang Lixia, Liu Qinglong, Wang Jianjun
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China.
Breed Sci. 2017 Dec;67(5):535-539. doi: 10.1270/jsbbs.16138. Epub 2017 Nov 15.
The fertility conversion of photoperiod thermo-sensitive genic male sterility (P/TGMS) lines in rice is mostly regulated by the P/TGMS genes in different environmental conditions. A point mutation with G-C on the () gene regulates the pollen fertility of Nongken58S and a large amount of Nongken58S-derived lines. In this study, we developed and designed a functional co-dominant marker according to the SNP loci for the () gene. We can differentiate the SNP loci in () gene from another TGMS lines and inbred cultivars using the dpms3-54 marker. The results showed that 376 bp band was detected in the homozygous genotype for (), while 359 bp band was detected in the homozygous genotype for non- (), two bands with 376 and 359 bp were detected in the heterozygous genotype. The dpms3-54 marker can be used to test the purity of two-line hybrid rice seeds and to divide each of F plant into homozygous and heterozygous genotypes at the seedling stage. Thus, this study provide a useful functional marker to detect () gene in different genetic resources and populations, which can be applied to the development and breeding of P/TGMS lines in two-line hybrid rice system with marker-assisted selection.
水稻光温敏核不育系（P/TGMS）在不同环境条件下的育性转换大多受P/TGMS基因调控。（）基因上一个G-C的点突变调控农垦58S及大量农垦58S衍生系的花粉育性。本研究根据（）基因的SNP位点开发并设计了一个功能性共显性标记。利用dpms3-54标记，我们可以区分（）基因中的SNP位点与其他温敏核不育系和自交品种。结果表明，在（）的纯合基因型中检测到376 bp条带，在非（）的纯合基因型中检测到359 bp条带，在杂合基因型中检测到376和359 bp两条带。dpms3-54标记可用于检测两系杂交水稻种子的纯度，并在苗期将F植株分为纯合和杂合基因型。因此，本研究提供了一个有用的功能性标记，用于检测不同遗传资源和群体中的（）基因，可应用于两系杂交水稻系统中P/TGMS系的开发和育种，并进行标记辅助选择。
