Abboud Talal George, Zubaer Abdullah, Wai Alvan, Hausner Georg
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Can J Microbiol. 2018 May;64(5):339-348. doi: 10.1139/cjm-2017-0605. Epub 2018 Feb 5.
Ophiostoma novo-ulmi, a member of the Ophiostomatales (Ascomycota), is the causal agent of the current Dutch elm disease pandemic in Europe and North America. The complete mitochondrial genome (mtDNA) of Ophiostoma novo-ulmi subsp. novo-ulmi, the European component of O. novo-ulmi, has been sequenced and annotated. Gene order (synteny) among the currently available members of the Ophiostomatales was examined and appears to be conserved, and mtDNA size variability among the Ophiostomatales is due in part to the presence of introns and their encoded open reading frames. Phylogenetic analysis of concatenated mitochondrial protein-coding genes yielded phylogenetic estimates for various members of the Ophiostomatales, with strong statistical support showing that mtDNA analysis may provide valuable insights into the evolution of the Ophiostomatales.
新榆枯萎病菌（Ophiostoma novo-ulmi）属于长喙壳目（子囊菌门），是当前欧洲和北美荷兰榆树病大流行的病原体。新榆枯萎病菌新榆亚种（Ophiostoma novo-ulmi subsp. novo-ulmi）是新榆枯萎病菌的欧洲类型，其完整的线粒体基因组（mtDNA）已完成测序和注释。对长喙壳目目前已知成员的基因顺序（同线性）进行了研究，结果表明其具有保守性，长喙壳目成员之间的线粒体DNA大小变异部分归因于内含子及其编码的开放阅读框的存在。对串联的线粒体蛋白质编码基因进行系统发育分析，得出了长喙壳目不同成员的系统发育估计值，有力的统计支持表明线粒体DNA分析可为长喙壳目的进化提供有价值的见解。
