Department of Human Genetics and Einthoven Laboratory for Experimental Medicine, Leiden University Medical Center (LUMC), 2300 RC Leiden, The Netherlands.
Department of Surgery and Einthoven Laboratory for Experimental Medicine, Leiden University Medical Center (LUMC), 2300 RC Leiden, The Netherlands.
Nutrients. 2018 Feb 3;10(2):172. doi: 10.3390/nu10020172.
The prebiotic inulin has proven effective at lowering inflammation and plasma lipid levels. As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic () mice. Male mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks. At week 3, a non-constrictive cuff was placed around the right femoral artery to induce accelerated atherosclerosis. At week 5, vascular pathology was determined by lesion thickness, vascular remodeling, and lesion composition. Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis. In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling. The lesions showed increased number of macrophages, smooth muscle cells, and collagen content. No effects on blood monocyte composition were found. Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure. In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic mice, accompanied by adverse lesion composition and outward remodeling. This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.
菊粉作为一种益生元已被证实可有效降低炎症和血浆脂质水平。动脉粥样硬化是由炎症和高脂血症共同引起的，因此，我们旨在确定菊粉补充对高脂血症（）小鼠动脉粥样硬化发展的影响。雄性 小鼠喂食高胆固醇（1%）饮食，同时补充或不补充 10%菊粉，持续 5 周。在第 3 周，在右股动脉周围放置非缩窄性袖带以诱导加速动脉粥样硬化。在第 5 周，通过病变厚度、血管重塑和病变成分来确定血管病理学。在整个研究过程中，测量血浆脂质，在第 5 周，使用流式细胞术分析确定血液单核细胞亚型。与我们的假设相反，菊粉加重了动脉粥样硬化的发展，表现为病变形成和血管向外重塑增加。病变中巨噬细胞、平滑肌细胞和胶原含量增加。未发现对血液单核细胞组成的影响。菊粉显著增加了血浆总胆固醇水平和总胆固醇暴露。总之，菊粉加重了高脂血症 小鼠加速动脉粥样硬化的发展，伴有不良的病变成分和向外重塑。这一过程并未伴有血液单核细胞组成的差异，表明加重的动脉粥样硬化发展是由血浆胆固醇增加驱动的。
