Perri Antonio, Gaida John H, Farina Andrea, Preda Fabrizio, Viola Daniele, Ballottari Matteo, Hauer Jürgen, De Silvestri Sandro, D'Andrea Cosimo, Cerullo Giulio, Polli Dario
Opt Express. 2018 Feb 5;26(3):2270-2279. doi: 10.1364/OE.26.002270.
We introduce a broadband single-pixel spectro-temporal fluorescence detector, combining time-correlated single photon counting (TCSPC) with Fourier transform (FT) spectroscopy. A birefringent common-path interferometer (CPI) generates two time-delayed replicas of the sample's fluorescence. Via FT of their interference signal at the detector, we obtain a two-dimensional map of the fluorescence as a function of detection wavelength and emission time, with high temporal and spectral resolution. Our instrument is remarkably simple, as it only requires the addition of a CPI to a standard single-pixel TCSPC system, and it shows a readily adjustable spectral resolution with inherently broad bandwidth coverage.
我们介绍了一种宽带单像素光谱-时间荧光探测器，它将时间相关单光子计数（TCSPC）与傅里叶变换（FT）光谱相结合。双折射共光路干涉仪（CPI）产生样品荧光的两个时间延迟副本。通过探测器处其干涉信号的傅里叶变换，我们获得了作为检测波长和发射时间函数的荧光二维图，具有高时间和光谱分辨率。我们的仪器非常简单，因为它只需要在标准单像素TCSPC系统中添加一个CPI，并且它具有易于调节的光谱分辨率和固有的宽带宽覆盖范围。
