Department of Anatomical, Histological, Forensic and Orthopaedic Sciences (SAIMLAL), Sapienza University of Rome, Viale Regina Elena 336, 00185, Rome, Italy.
Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Sapienza University of Rome, Via di Grottarossa 1035, 00189, Rome, Italy.
Sci Rep. 2018 Feb 5;8(1):2387. doi: 10.1038/s41598-018-20699-1.
Traumatic brain injury (TBI) is one of the most important death and disability cause, involving substantial costs, also in economic terms, when considering the young age of the involved subject. Aim of this paper is to report a series of patients treated at our institutions, to verify neurological results at six months or survival; in fatal cases we searched for βAPP, GFAP, IL-1β, NFL, Spectrin II, TUNEL and miR-21, miR-16, and miR-92 expressions in brain samples, to verify DAI diagnosis and grade as strong predictor of survival and inflammatory response. Concentrations of 8OHdG as measurement of oxidative stress was performed. Immunoreaction of β-APP, IL-1β, GFAP, NFL, Spectrin II and 8OHdG were significantly increased in the TBI group with respect to control group subjects. Cell apoptosis, measured by TUNEL assay, were significantly higher in the study group than control cases. Results indicated that miR-21, miR-92 and miR-16 have a high predictive power in discriminating trauma brain cases from controls and could represent promising biomarkers as strong predictor of survival, and for the diagnosis of postmortem traumatic brain injury.
创伤性脑损伤（TBI）是最重要的死亡和残疾原因之一，涉及大量成本，尤其是考虑到所涉及的年轻患者。本文的目的是报告在我们机构治疗的一系列患者，以验证六个月时的神经学结果或存活率；在致命病例中，我们在脑组织样本中寻找βAPP、GFAP、IL-1β、NFL、Spectrin II、TUNEL 和 miR-21、miR-16 和 miR-92 的表达，以验证弥漫性轴索损伤（DAI）的诊断和分级作为存活率和炎症反应的强预测因子。还进行了 8OHdG 浓度（氧化应激的测量）。与对照组相比，TBI 组的β-APP、IL-1β、GFAP、NFL、Spectrin II 和 8OHdG 的免疫反应显著增加。TUNEL 检测表明，研究组的细胞凋亡明显高于对照组。结果表明，miR-21、miR-92 和 miR-16 具有较高的预测能力，可以区分创伤性脑损伤病例与对照组，并可能成为存活率的强预测因子和死后创伤性脑损伤的诊断标志物。
