Jia Ling-Han, Liu Yi, Li Yu-Zhen
School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
Department of Pharmacy, Peking University People's Hospital, Beijing 100044, China.
J Pharm Anal. 2011 Aug;1(3):213-218. doi: 10.1016/j.jpha.2011.04.002. Epub 2011 Jul 22.
The total volatile components were extracted from safflower by ultrasonic-assisted solvent extraction (USE) and their chemical constituents were analyzed by gas chromatography-mass spectrometry (GC-MS) to provide scientific basis for the quality control of safflower. Five different solvents (diethyl ether, ethanol, ethyl acetate, dichloromethane and acetone) were used and compared in terms of number of volatile components extracted and the peak areas of these components in TIC. The results showed that USE could be used as an efficient and rapid method for extracting the volatile components from safflower. It also could be found that the number of components in the TIC of ethyl acetate extract was more than that in the TIC of other solvent ones. Meanwhile, the volatile components of safflower from Xinjiang Autonomous Region and Henan Province of China were different in chemical components and relative contents. It could be concluded that both the extraction solvents and geographical origin of safflower are responsible for these differences. The experimental results also indicated that USE/GC-MS is a simple, rapid and effective method to analyze the volatile oil components of safflower.
采用超声辅助溶剂萃取法（USE）从红花中提取总挥发性成分，并通过气相色谱-质谱联用（GC-MS）对其化学成分进行分析，为红花的质量控制提供科学依据。使用了五种不同的溶剂（乙醚、乙醇、乙酸乙酯、二氯甲烷和丙酮），并就提取的挥发性成分数量以及这些成分在总离子流色谱图（TIC）中的峰面积进行了比较。结果表明，超声辅助溶剂萃取法可作为从红花中提取挥发性成分的一种高效、快速的方法。还发现乙酸乙酯提取物的总离子流色谱图中的成分数量多于其他溶剂提取物的总离子流色谱图中的成分数量。同时，中国新疆自治区和河南省的红花挥发性成分在化学成分和相对含量上存在差异。可以得出结论，提取溶剂和红花的地理来源都是造成这些差异的原因。实验结果还表明，超声辅助溶剂萃取法/气相色谱-质谱联用是分析红花挥发油成分的一种简单、快速且有效的方法。
