基于磁性微粒的化学发光酶免疫分析法与传统比色酶联免疫吸附测定法检测血清甲胎蛋白的比较
Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein.
作者信息
Zhang Qian-Yun, Chen Hui, Lin Zhen, Lin Jin-Ming
机构信息
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100029, China.
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
J Pharm Anal. 2012 Apr;2(2):130-135. doi: 10.1016/j.jpha.2011.10.001. Epub 2012 Jan 27.
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better linearity, recovery, precision, sensitivity and validity. AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA, and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit. The correlation coefficient between MPs-CLEIA and ELISA was obtained with =0.6703; however, the correlation between MPs-CLEIA and ECLIA (=0.9582) was obviously better than that between colorimetric ELISA and ECLIA (=0.6866).
开发了一种基于磁性微粒的化学发光酶免疫分析方法（MmPs-CLEIA），用于与传统比色酶联免疫吸附测定法（ELISA）并行评估血清甲胎蛋白（AFP）。MmPs-CLEIA与比色ELISA之间的系统比较得出结论，磁性微粒化学发光酶免疫分析方法使用的免疫试剂剂量更少，总检测时间更短，并且线性、回收率、精密度、灵敏度和有效性更好。通过所提出的磁性微粒化学发光酶免疫分析方法和ELISA对40份人血清样本中的AFP进行了检测，并将结果与商业电化学发光免疫分析（ECLIA）试剂盒进行了比较。磁性微粒化学发光酶免疫分析方法与ELISA之间的相关系数为r = 0.6703；然而，磁性微粒化学发光酶免疫分析方法与ECLIA之间的相关性（r = 0.9582）明显优于比色ELISA与ECLIA之间的相关性（r = 0.6866）。
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