Al Abachi Mouayed Q, Hadi Hind
Department of Chemistry, College of Science, University of Baghdad, Jadriyah, Baghdad, Iraq.
J Pharm Anal. 2012 Oct;2(5):350-355. doi: 10.1016/j.jpha.2012.04.005. Epub 2012 Apr 25.
Simple and sensitive normal and reverse flow injection methods for spectrophotometric determination of thiamine hydrochloride (THC) at the microgram level were proposed and optimized. Both methods are based on the reaction between THC and diazotized metoclopramide in alkaline medium. Beer's law was obeyed over the range of 10-300 and 2-90 μg/mL, the limits of detection were 2.118 and 0.839 μg/mL and the sampling rates were 80 and 95 injections per hour for normal and reverse flow injection methods respectively. The application of both methods to commercially available pharmaceuticals produced acceptable results. The flow system is suitable for application in quality control processes.
提出并优化了用于分光光度法在微克水平测定盐酸硫胺素（THC）的简单且灵敏的正向和反向流动注射方法。两种方法均基于THC与重氮化甲氧氯普胺在碱性介质中的反应。在10 - 300和2 - 90μg/mL范围内符合比尔定律，正向和反向流动注射方法的检测限分别为2.118和0.839μg/mL，采样率分别为每小时80次和95次进样。两种方法应用于市售药品均得到了可接受的结果。该流动系统适用于质量控制过程。
