Jirikowski G F, Merchenthaler I, Rieger G E, Stumpf W E
Neurosci Lett. 1986 Apr 11;65(2):121-6. doi: 10.1016/0304-3940(86)90290-9.
Combined autoradiography and immunocytochemistry was performed to examine estradiol-concentrating neurons of the arcuate nucleus in the hypothalamus of ovariectomized rats and mice for beta-endorphin-like immunoreactivity. A high number of [3H]estradiol-labeled cells occurred in the arcuate nucleus of both species. Neurons immunoreactive for beta-endorphin could be visualized throughout the arcuate nucleus as well as ventrolaterally outside of this nucleus. Colocalization of [3H] estradiol nuclear labeling and beta-endorphin-like cytoplasmic immunoreactivity could be found scattered throughout the arcuate nucleus, most frequently in the ventromedial part, although most of the [3H]estradiol target neurons did not react with the antibodies. The results suggest that a subpopulation of beta-endorphin neurons is directly addressed by estradiol and that the arcuate nucleus contains different groups of estradiol receptive neurosecretory cells.
采用放射自显影和免疫细胞化学相结合的方法，对去卵巢大鼠和小鼠下丘脑弓状核中集中雌二醇的神经元进行β-内啡肽样免疫反应性检测。在这两个物种的弓状核中均出现了大量[3H]雌二醇标记的细胞。整个弓状核以及该核外侧腹侧均可观察到对β-内啡肽呈免疫反应的神经元。[3H]雌二醇核标记与β-内啡肽样细胞质免疫反应性的共定位可见于整个弓状核，最常见于腹内侧部分，尽管大多数[3H]雌二醇靶神经元不与抗体发生反应。结果表明，雌二醇可直接作用于β-内啡肽神经元的一个亚群，且弓状核含有不同组别的雌二醇感受性神经分泌细胞。
