Dong Huike, Lu Guanghua, Yan Zhenhua, Liu Jianchao, Yang Haohan, Nkoom Matthew
Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, 210098, Nanjing, China.
Water Conservancy Project & Civil Engineering College, Tibet Agriculture & Animal Husbandry University, 860000, Linzhi, China.
Ecotoxicology. 2018 Apr;27(3):313-324. doi: 10.1007/s10646-018-1896-6. Epub 2018 Feb 5.
As a cycloaliphatic brominated flame retardant, hexabromocyclododecane (HBCD) has been widely used in building thermal insulation and fireproof materials. However, there is little information on the bioconcentration as well as effects with respect to HBCD exposure in the aquatic environment. To investigate the bioconcentration of HBCD in tissues (muscle and liver) and its biochemical and behavioural effects, juvenile crucian carp (Carassius auratus) were exposed to different concentrations of technical HBCD (nominal concentrations, 2, 20, 200 μg/L) for 7 days, using a flow-through exposure system. HBCD was found to concentrate in the liver and muscle with a terminal concentration of 0.60 ± 0.22 μg/g lw (lipid weight) and 0.18 ± 0.02 μg/g lw, respectively, at an environmentally-relevant concentration (2 μg/L). The total thyroxine and total triiodothyronine in the fish plasma were lowered as a result of exposure to the HBCD. Acetylcholinesterase activity in the brain was increased, while swimming activity was inhibited and shoaling inclination was enhanced after exposure to 200 μg/L HBCD. Feeding rate was suppressed in the 20 and 200 μg/L treatment groups. In summary, HBCD concentrations 10-100× higher than the current environmentally-relevant exposures induced adverse effects in the fish species tested in this study. These results suggest that increasing environmental concentrations and/or species with higher sensitivity than carp might be adversely affected by HBCD.
作为一种脂环族溴化阻燃剂，六溴环十二烷（HBCD）已广泛应用于建筑保温和防火材料中。然而，关于HBCD在水生环境中的生物富集以及暴露影响的信息却很少。为了研究HBCD在组织（肌肉和肝脏）中的生物富集及其生化和行为影响，使用流水暴露系统，将幼龄鲫鱼（Carassius auratus）暴露于不同浓度的工业级HBCD（标称浓度为2、20、200μg/L）中7天。发现在环境相关浓度（2μg/L）下，HBCD在肝脏和肌肉中富集，终浓度分别为0.60±0.22μg/g lw（脂重）和0.18±0.02μg/g lw。暴露于HBCD导致鱼血浆中的总甲状腺素和总三碘甲状腺原氨酸降低。暴露于200μg/L HBCD后，脑中乙酰胆碱酯酶活性增加，而游泳活动受到抑制，集群倾向增强。在20和200μg/L处理组中摄食率受到抑制。总之，本研究中测试的鱼类物种在HBCD浓度比当前环境相关暴露高10 - 100倍时会产生不利影响。这些结果表明，环境浓度的增加和/或比鲤鱼敏感性更高的物种可能会受到HBCD的不利影响。
