Holmberg J T, Hultberg B, Hägerstrand I, Ihse I, Isaksson A
Scand J Gastroenterol. 1986 Mar;21(2):205-14. doi: 10.3109/00365528609034648.
Levels of the lysosomal enzymes beta-glucuronidase and beta-hexosaminidase were determined in tissues from rats at 1-7 weeks after permanent bile duct occlusion (28 animals) and sham operation (16 animals). With increasing duration of bile duct occlusion, increasing levels were found in the liver and spleen, whereas levels were unaffected in the pancreas, kidneys, brain, muscle, and skin. Histochemical investigation showed that the lysosomal enzymes accumulated in the hepatocytes and in the increasing numbers of reticuloendothelial cells of the liver and spleen during cholestasis. No significant accumulation of lysosomal enzymes was seen in the pancreas. In preparations of isolated hepatocytes and non-parenchymal cells increased specific activity of lysosomal enzymes in cholestasis appeared in both cellular fractions. It is assumed that the serum increase of lysosomal enzymes in late cholestasis is partly due to a release from activated macrophages in the liver and spleen.
在永久性胆管结扎术后1至7周的大鼠组织（28只动物）和假手术组（16只动物）中测定了溶酶体酶β-葡萄糖醛酸酶和β-己糖胺酶的水平。随着胆管结扎时间的延长，肝脏和脾脏中的酶水平升高，而胰腺、肾脏、大脑、肌肉和皮肤中的酶水平未受影响。组织化学研究表明，胆汁淤积期间，溶酶体酶在肝细胞以及肝脏和脾脏中数量不断增加的网状内皮细胞中积累。在胰腺中未观察到溶酶体酶的显著积累。在分离的肝细胞和非实质细胞制剂中，胆汁淤积时溶酶体酶的比活性在两个细胞组分中均升高。据推测，晚期胆汁淤积时血清溶酶体酶升高部分归因于肝脏和脾脏中活化巨噬细胞的释放。
