Department of Chemistry, Veer Surendra Sai University of Technology, Sambalpur 768018, Odisha, India.
Department of Chemistry, Veer Surendra Sai University of Technology, Sambalpur 768018, Odisha, India.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:130-141. doi: 10.1016/j.msec.2017.11.028. Epub 2017 Dec 13.
Herein, novel biodegradable, stimuli responsive, chemically cross-linked and porous polyacrylamide/dextran (PAM/D) nanohydrogels hybrid composites are synthesized by in situ polymerization technique with incorporation of reduced nano silver. The interaction of nano silver with PAM in presence of dextran is investigated by Fourier transforms infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies. The elemental composition of the hybrid nanohydrogels is studied by X-ray photoelectron spectroscopy (XPS) whereas; the surface morphology of nanohydrogels hybrid composites is studied by field emission scanning electron microscope (FESEM) by which, it is observed that, the silver nanoparticles are homogeneously dispersed throughout the nanohydrogel network. From high resolution transmission electron microscopy (HRTEM), the average size of silver nanoparticles is found to be 20nm. The swelling, deswelling and water retention properties of nanohydrogels hybrid composites are measured in order to investigate the release rate of the ornidazole drugs. The in vitro release rate of ornidazole drugs is found to be 98.5% in 6h. The antibacterial activities and the cytotoxicity tests along with positive and negative control of hybrid nanohydrogels are investigated. The loss modulus, gain modulus and complex viscosities are determined from rheological behaviour of the nanohydrogels. It is found that, the value of tanδ varies from 0.1 to 0.8. Nano silver decorated PAM/D nanohydrogels are stable, nontoxic with antibacterial behaviour may be suitable for drugs delivery vehicle.
在此，通过原位聚合技术合成了新型的可生物降解、刺激响应、化学交联和多孔的聚丙烯酰胺/葡聚糖（PAM/D）纳米水凝胶杂化复合材料，并掺入了还原纳米银。通过傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）研究了纳米银与 PAM 在葡聚糖存在下的相互作用。通过 X 射线光电子能谱（XPS）研究了杂化纳米水凝胶的元素组成，而纳米水凝胶杂化复合材料的表面形态则通过场发射扫描电子显微镜（FESEM）进行研究，观察到银纳米粒子均匀地分散在纳米水凝胶网络中。从高分辨率透射电子显微镜（HRTEM）中，发现银纳米粒子的平均尺寸为 20nm。测量了纳米水凝胶杂化复合材料的溶胀、去溶胀和保水性能，以研究奥硝唑药物的释放速率。在 6 小时内，奥硝唑药物的体外释放率达到 98.5%。还研究了杂化纳米水凝胶的抗菌活性和细胞毒性测试以及阳性和阴性对照。通过纳米水凝胶的流变行为确定了损耗模量、增益模量和复合粘度。结果发现，tanδ 值从 0.1 变化到 0.8。纳米银修饰的 PAM/D 纳米水凝胶具有稳定性、无毒性和抗菌行为，可能适合作为药物输送载体。
