Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India.
Central Animal Lab Facility, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:239-248. doi: 10.1016/j.msec.2018.01.001. Epub 2018 Jan 10.
The treatment of critical sized bone defect remains a significant challenge in orthopedics. The objective of the study is to evaluate the effect of the combination of bone morphogenetic protein 2 (BMP2) expressing genetically engineered mesenchymal stem cells (MSCs) [MSCs engineered using a multimam vector, pAceMam1, an emerging gene delivery vector] and an osteoconductive scaffold [silica coated nanohydroxyapatite-gelatin reinforced with fibers] in enhancing bone regeneration in critical sized segmental defects. The scaffold with transfected MSCs showed significantly higher viability, proliferation and osteogenic differentiation in vitro. Further, this group augmented union and new bone formation in critical sized rat femoral segmental defect at 12 weeks when compared to control groups (scaffold with MSCs and scaffold alone). These data demonstrated that the MSCs engineered for transient expression of BMP2 can improve the repair of segmental defects, which paves an avenue for using pAceMam1 as a vector for bone tissue regeneration.
治疗临界尺寸骨缺损仍然是骨科领域的一个重大挑战。本研究的目的是评估骨形态发生蛋白 2（BMP2）表达的基因工程间充质干细胞（MSCs）[使用多顺反子载体 pAceMam1 工程化的 MSCs，一种新兴的基因传递载体]与骨传导支架[二氧化硅涂层纳米羟基磷灰石-纤维增强明胶]联合应用在增强临界尺寸节段性缺损骨再生中的效果。转染 MSC 的支架在体外表现出更高的活力、增殖和成骨分化。此外，与对照组（MSC 支架和单独支架）相比，该组在 12 周时增强了大鼠股骨临界尺寸节段性缺损的愈合和新骨形成。这些数据表明，用于 BMP2 瞬时表达的工程 MSC 可以改善节段性缺损的修复，为使用 pAceMam1 作为骨组织再生载体开辟了道路。
