Kaczmarek L, Ferguson B, Rosenberg M, Baserga R
Virology. 1986 Jul 15;152(1):1-10. doi: 10.1016/0042-6822(86)90366-1.
The purified Escherichia coli-expressed products of the human subgroup-C adenovirus E1A 13 S and 12 S mRNAs are shown to induce cellular DNA synthesis when introduced by microinjection into quiescent, G0-arrested mammalian cells from immortalized cell lines. The E1A proteins stimulated cellular DNA synthesis in mouse Swiss 3T3 cells, when microinjected either individually or in combination. A truncated E1A protein, in which 169 carboxyl terminal residues of the 289-amino acid E1A 13 S mRNA product are deleted, was unable to induce cellular DNA synthesis in these cells. Our results provide evidence that E1A proteins can function, independent of other viral functions, in the stimulation of cellular DNA synthesis in certain cell types. The present results are consistent with the E1A gene products acting to modulate the expression of the cellular genes which control cell cycle progression into S phase.
人C亚组腺病毒E1A 13S和12S信使核糖核酸经纯化的大肠杆菌表达产物，在通过显微注射导入来自永生细胞系的静止、处于G0期阻滞的哺乳动物细胞时，显示出能诱导细胞DNA合成。当单独或联合显微注射时，E1A蛋白能刺激小鼠瑞士3T3细胞中的细胞DNA合成。一种截短的E1A蛋白，其289个氨基酸的E1A 13S信使核糖核酸产物的169个羧基末端残基被缺失，在这些细胞中无法诱导细胞DNA合成。我们的结果提供了证据，表明E1A蛋白在某些细胞类型中能独立于其他病毒功能，发挥刺激细胞DNA合成的作用。目前的结果与E1A基因产物调节控制细胞周期进入S期的细胞基因表达的作用是一致的。
