Stabel S, Argos P, Philipson L
EMBO J. 1985 Sep;4(9):2329-36. doi: 10.1002/j.1460-2075.1985.tb03934.x.
The induction of DNA synthesis in growth-arrested mouse fibroblasts (NIH 3T3) was studied by microinjection of different constructs of adenovirus DNA using SV40 DNA and plasmid DNA as positive and negative controls. The E1A region of adenovirus types 2 and 12 appears to be sufficient to induce cellular DNA synthesis after growth arrest in approximately 30% of the cells and both 13S and 12S cDNA constructs mediate this effect. The presence of the E1A protein products as assayed by immunofluorescence does not strictly correlate with the induction of DNA synthesis in microinjected cells in contrast to the SV40 large T-antigen. Microinjection of truncated fragments of the Ad12 E1A region suggests, however, that the protein products of 12S and 13S may be involved in the induction process. A sequence comparison of the SV40 T-antigen and the adenovirus E1A products identified a region of significant homology providing a basis for a hypothesis concerning the evolution of T-antigen genes in DNA viruses.
通过显微注射不同构建体的腺病毒DNA，以SV40 DNA和质粒DNA作为阳性和阴性对照，研究了生长停滞的小鼠成纤维细胞（NIH 3T3）中DNA合成的诱导情况。2型和12型腺病毒的E1A区域似乎足以在约30%的细胞生长停滞后诱导细胞DNA合成，并且13S和12S cDNA构建体均介导这种效应。与SV40大T抗原相反，通过免疫荧光检测到的E1A蛋白产物的存在与显微注射细胞中DNA合成的诱导并不严格相关。然而，对Ad12 E1A区域截短片段的显微注射表明，12S和13S的蛋白产物可能参与了诱导过程。SV40 T抗原和腺病毒E1A产物的序列比较确定了一个显著同源区域，为关于DNA病毒中T抗原基因进化的假说提供了基础。
