Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
Department of Dental Hygiene, Daegu Health College, Daegu, Republic of Korea.
Arch Oral Biol. 2018 Apr;88:42-46. doi: 10.1016/j.archoralbio.2018.01.009.
The biofilm of Streptococcus mutans is associated with induction of dental caries. Also, they produce glucan as an extracellular polysaccharide through glucosyltransferases and help the formation of cariogenic biofilm. β-caryophyllene has been used for therapeutic agent in traditional medicine and has antimicrobial activity. The purpose of this study was to investigate the effect of β-caryophyllene on S. mutans biofilm and the expression of biofilm-related factor.
The susceptibility assay of S. mutans for β-caryophyllene was performed to investigate inhibitory concentration for S. mutans growth. To evaluated the effect of β-caryophyllene on S. mutans biofilm, β-caryophyllene was treated on S. mutans in the various concentrations before or after the biofilm formation. Live S. mutans in the biofilm was counted by inoculating on Mitis-salivarius agar plate, and S. mutans biofilm was analyzed by confocal laser scanning microscope after staining bacterial live/dead staining kit. Finally, the expression of glucosyltransferases of S. mutans was investigated by real-time RT-PCR after treating with β-caryophyllene at the non-killing concentration of S. mutans.
The growth of S. mutans was inhibited by β-caryophyllene in above concentration of 0.078%, S. mutans biofilm was inhibited by β-caryophyllene in above 0.32%. Also, 2.5% of β-caryophyllene showed anti-biofilm activity for S. mutans biofilm. β-caryophyllene reduced the expression of gtf genes at a non-killing concentration for S. mutans. On the basis on these results, β-caryophyllene may have anti-biofilm activity and the inhibitory effect on biofilm related factor.
β-caryophyllene may inhibit cariogenic biofilm and may be a candidate agent for prevention of dental caries.
变形链球菌的生物膜与龋齿的诱导有关。此外，它们通过葡糖基转移酶产生作为细胞外多糖的葡聚糖，有助于致龋生物膜的形成。β-石竹烯已被传统医学用作治疗剂，具有抗菌活性。本研究旨在研究β-石竹烯对变形链球菌生物膜和生物膜相关因子表达的影响。
为了研究β-石竹烯对变形链球菌生长的抑制浓度，对变形链球菌进行了β-石竹烯敏感性测定。为了评估β-石竹烯对变形链球菌生物膜的影响，在生物膜形成之前或之后，用不同浓度的β-石竹烯处理变形链球菌。通过接种在米氏链球菌琼脂平板上，计算生物膜中活变形链球菌的数量，并在染色细菌死活染色试剂盒后，通过共聚焦激光扫描显微镜分析变形链球菌生物膜。最后，在非致死浓度的β-石竹烯处理后，通过实时 RT-PCR 研究变形链球菌葡糖基转移酶的表达。
浓度高于 0.078%的β-石竹烯抑制变形链球菌的生长，浓度高于 0.32%的β-石竹烯抑制变形链球菌生物膜的形成。此外，2.5%的β-石竹烯对变形链球菌生物膜具有抗生物膜活性。β-石竹烯在不杀死变形链球菌的浓度下降低 gtf 基因的表达。基于这些结果，β-石竹烯可能具有抗生物膜活性和抑制生物膜相关因子的作用。
β-石竹烯可能抑制致龋生物膜，是预防龋齿的候选药物。
