Kim Young-Jae, Lee Sung-Hoon
Department of Pediatric Dentistry, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea.
Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan 31116, Republic of Korea.
J Microbiol Biotechnol. 2016 Nov 28;26(11):1829-1835. doi: 10.4014/jmb.1604.04008.
Dental caries is caused by cariogenic biofilm, an oral biofilm including . Recently, the prevention of dental caries using various probiotics has been attempted. HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of HY 449 on cariogenic biofilm and to analyze its inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of HY 449 and ATCC 19435, and analyzed with a confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both strains, and HY 449 exhibited more inhibitory effects than ATCC 19435. In order to analyze and to compare the inhibitory mechanisms, the antibacterial activity of the spent culture medium from both strains against was investigated, and the expression of glucosyltransferases () of was then analyzed by real-time RT-PCR. In addition, the sucrose fermentation ability of both strains was examined. Both strains showed antibacterial activity and inhibited the expression of , and the difference between both strains did not show. In the case of sucrose-fermenting ability, HY 449 fermented sucrose but ATCC 19435 did not. HY 449 inhibited the uptake of sucrose and the expression of , whereby the development of cariogenic biofilm may be inhibited. In conclusion, HY 449 may be a useful probiotic for the prevention of dental caries.
龋齿是由致龋生物膜引起的，致龋生物膜是一种口腔生物膜。最近，人们尝试使用各种益生菌来预防龋齿。HY 449是一种益生菌。本研究的目的是研究HY 449对致龋生物膜的影响并分析其抑制机制。在有或没有HY 449和ATCC 19435的情况下形成致龋生物膜，并用共聚焦激光显微镜进行分析。在添加了这两种菌株的培养物中，致龋生物膜的形成减少，并且HY 449比ATCC 19435表现出更强的抑制作用。为了分析和比较抑制机制，研究了这两种菌株的用过的培养基对（此处原文缺失被抑制对象）的抗菌活性，然后通过实时逆转录聚合酶链反应分析（此处原文缺失被分析对象）的葡糖基转移酶（）的表达。此外，检测了这两种菌株的蔗糖发酵能力。两种菌株均表现出抗菌活性并抑制（此处原文缺失被抑制对象）的表达，并且两种菌株之间没有差异。在蔗糖发酵能力方面，HY 449能发酵蔗糖而ATCC 19435不能。HY 449抑制蔗糖的摄取和（此处原文缺失被抑制对象）的表达，从而可能抑制致龋生物膜的形成。总之，HY 449可能是一种预防龋齿的有用益生菌。
