Marchut E, Gumińska M, Kedryna T
Acta Biochim Pol. 1986;33(1):7-16.
Inhibition of glycolysis in Ehrlich ascites tumour cells by saturated fatty acids, added either in form of potassium salts or incorporated into phosphatidylcholine liposomes, increases with the increasing carbon atom chain length and is independent of the concentration within the range of 0.1 to 1.0 mM. In contrast, the inhibition of glycolysis in the cytosolic fraction from Ehrlich ascites cells depends on the concentration of fatty acids. The content of ATP in Ehrlich ascites cells incubated with fatty acids increases with increasing carbon atom chain length, which leads to a crossing-over in the concentrations of pyruvate and 2-phosphoenolpyruvate. Lowering of the sum of both these metabolites by palmitate and stearate points to the inhibition not only of pyruvate kinase but also of other enzymes of early steps of glycolysis. Fatty acids in intact Ehrlich ascites cells inhibit all three key glycolytic enzymes but added to the cytosolic fraction affect mainly the activity of phosphofructokinase. The inhibition of pyruvate kinase by fatty acids is smaller in the cytosolic fraction from tumour cells than from liver and muscles.
以钾盐形式添加或掺入磷脂酰胆碱脂质体中的饱和脂肪酸对艾氏腹水瘤细胞糖酵解的抑制作用，随碳原子链长度的增加而增强，且在0.1至1.0 mM范围内与浓度无关。相比之下，艾氏腹水细胞胞质部分糖酵解的抑制作用取决于脂肪酸的浓度。用脂肪酸孵育的艾氏腹水细胞中ATP的含量随碳原子链长度的增加而增加，这导致丙酮酸和2-磷酸烯醇丙酮酸浓度的交叉。棕榈酸和硬脂酸使这两种代谢物的总量降低，这表明不仅丙酮酸激酶受到抑制，糖酵解早期步骤的其他酶也受到抑制。完整艾氏腹水细胞中的脂肪酸抑制所有三种关键糖酵解酶，但添加到胞质部分时主要影响磷酸果糖激酶的活性。脂肪酸对肿瘤细胞胞质部分丙酮酸激酶的抑制作用比对肝脏和肌肉的抑制作用小。
