Tan Kok Keong, Bay Boon Huat, Gopalakrishnakone Ponnampalam
Department of Anatomy, National University of Singapore, 4 Medical Drive, MD10, Singapore, 117594, Singapore.
Department of Anatomy, National University of Singapore, 4 Medical Drive, MD10, Singapore, 117594, Singapore; Health & Social Sciences Cluster, Singapore Institute of Technology, 10 Dover Drive, Singapore, 138683, Singapore.
Toxicon. 2018 Mar 15;144:7-13. doi: 10.1016/j.toxicon.2018.01.015. Epub 2018 Feb 4.
L-amino acid oxidase (LAAO) is a protein toxin commonly found in snake venom. It has many applications, ranging from biotechnology to potential anticancer therapeutics. LAAO converts L-amino acid into α-keto acid and release ammonia and hydrogen peroxide as by-products. Induction of oxidative stress in cancer cells is one of the cancer treatment strategies as controlled and targeted release of hydrogen peroxide can theoretically induce sufficient oxidative stress to kill cancer cells. Furthermore, L-amino acid oxidase has been shown to selectively bind to cell membranes of specific phospholipid composition and deliver the hydrogen peroxide to localized regions on the cell surface. In this mini review, we discuss the relevance of L-amino acid oxidase, in terms of its structure and enzyme activity, its potential as a cytotoxic agent and exploitation of its cytotoxic nature as an anticancer therapeutic.
L-氨基酸氧化酶（LAAO）是一种常见于蛇毒中的蛋白质毒素。它有许多应用，从生物技术到潜在的抗癌治疗。LAAO将L-氨基酸转化为α-酮酸，并释放氨和过氧化氢作为副产物。诱导癌细胞中的氧化应激是癌症治疗策略之一，因为理论上过氧化氢的可控和靶向释放可以诱导足够的氧化应激来杀死癌细胞。此外，L-氨基酸氧化酶已被证明能选择性地结合特定磷脂组成的细胞膜，并将过氧化氢输送到细胞表面的局部区域。在本综述中，我们从其结构和酶活性、作为细胞毒性剂的潜力以及利用其细胞毒性性质作为抗癌治疗等方面讨论L-氨基酸氧化酶的相关性。
