Laurent Cédric P, Vaquette Cédryck, Liu Xing, Schmitt Jean-François, Rahouadj Rachid
1 CNRS, LEMTA, UMR 7563, Université de Lorraine, France.
2 95541 Queensland University of Technology (QUT) , Brisbane, Australia.
J Biomater Appl. 2018 Apr;32(9):1276-1288. doi: 10.1177/0885328218757064. Epub 2018 Feb 6.
Poly(lactide-co-ε-caprolactone) (PLCL) has been reported to be a good candidate for tissue engineering because of its good biocompatibility. Particularly, a braided PLCL scaffold (PLL/PCL ratio = 85/15) has been recently designed and partially validated for ligament tissue engineering. In the present study, we assessed the in vivo biocompatibility of acellular and cellularised scaffolds in a rat model. We then determined its in vitro biocompatibility using stem cells issued from both bone marrow and Wharton Jelly. From a biological point of view, the scaffold was shown to be suitable for tissue engineering in all these cases. Secondly, while the initial mechanical properties of this scaffold have been previously reported to be adapted to load-bearing applications, we studied the evolution in time of the mechanical properties of PLCL fibres due to hydrolytic degradation. Results for isolated PLCL fibres were extrapolated to the fibrous scaffold using a previously developed numerical model. It was shown that no accumulation of plastic strain was to be expected for a load-bearing application such as anterior cruciate ligament tissue engineering. However, PLCL fibres exhibited a non-expected brittle behaviour after two months. This may involve a potential risk of premature failure of the scaffold, unless tissue growth compensates this change in mechanical properties. This combined study emphasises the need to characterise the properties of biomaterials in a pluridisciplinary approach, since biological and mechanical characterisations led in this case to different conclusions concerning the suitability of this scaffold for load-bearing applications.
聚（丙交酯-共-ε-己内酯）（PLCL）因其良好的生物相容性，已被报道是组织工程的良好候选材料。特别是，一种编织的PLCL支架（PLL/PCL比例 = 85/15）最近已被设计出来，并在韧带组织工程中得到了部分验证。在本研究中，我们在大鼠模型中评估了脱细胞和细胞化支架的体内生物相容性。然后，我们使用来自骨髓和沃顿胶的干细胞测定了其体外生物相容性。从生物学角度来看，在所有这些情况下，该支架都显示出适用于组织工程。其次，虽然该支架的初始力学性能先前已被报道适用于承重应用，但我们研究了由于水解降解导致的PLCL纤维力学性能随时间的演变。使用先前开发的数值模型将分离的PLCL纤维的结果外推到纤维支架上。结果表明，对于诸如前交叉韧带组织工程这样的承重应用，预计不会有塑性应变的积累。然而，两个月后PLCL纤维表现出意外的脆性。这可能涉及支架过早失效的潜在风险，除非组织生长能够补偿这种力学性能的变化。这项综合研究强调了以多学科方法表征生物材料特性的必要性，因为在这种情况下，生物学和力学表征就该支架在承重应用中的适用性得出了不同的结论。
