Laboratory of Protozoology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP 05403-000, Brazil.
Laboratory of Vectors, Superintendence for the Health Survaillance of Amapá, Macapá, AP 68905-230, Brazil.
Viruses. 2018 Nov 24;10(12):666. doi: 10.3390/v10120666.
Classical insect-specific flaviviruses (cISFs) have been widely detected in different countries in the last decades. Here, we characterize the near full-length genomes of two cISFs detected in mosquitoes collected in the city of Macapá, state of Amapá, Amazon region of Brazil. A total of 105 pools of female mosquitos were analyzed by next-generation sequencing (NGS). Comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (CFAV) and two of Culex flavivirus (CxFV). All sequences were obtained from pools of Culex sp., except for one sequence of CFAV detected in a pool of Aedes aegypti. Both CxFV strains are phylogenetically related to a strain isolated in 2012 in the Southeast region of Brazil. The CFAV strains are the first of this species to be identified in Brazil and one of them is highly divergent from other strains of CFAV that have been detected worldwide. In conclusion, CFAV and CxFV, circulate in mosquitoes in Brazil. One strain of CFAV is highly divergent from others previously described, suggesting that a novel strain of CFAV is present in this region.
在过去几十年中，经典的昆虫特异性黄病毒（cISFs）已在许多国家广泛检测到。在这里，我们对在巴西亚马孙地区阿马帕州马卡帕市采集的蚊子中检测到的两种 cISF 的近全长基因组进行了特征描述。通过下一代测序（NGS）对总共 105 个雌性蚊子进行了分析。比较基因组学和系统发育分析确定了三种细胞融合剂病毒（CFAV）和两种库蚊黄病毒（CxFV）的菌株。除了在一只埃及伊蚊的一个蚊群中检测到的一种 CFAV 序列外，所有序列均来自库蚊蚊群获得。两种 CxFV 菌株在系统发育上与 2012 年在巴西东南部分离的一株有关。鉴定出的 CFAV 株系是巴西首次发现的该种病毒，其中一种与已在全球检测到的其他 CFAV 株系高度分化。总之，CFAV 和 CxFV 在巴西的蚊子中传播。一种 CFAV 株系与其他先前描述的株系高度分化，表明该地区存在一种新型 CFAV 株系。
