Unidade de Microbiologia Médica, Grupo de Virologia, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
Virus Res. 2012 Aug;167(2):152-61. doi: 10.1016/j.virusres.2012.04.010. Epub 2012 Apr 28.
We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs. The CTFV genome encodes a single polyprotein with 3357 residues showing all the features expected for those of flaviviruses. Phylogenetic analyses based on all ISF sequences available to date, place CTFV among Culex-associated flaviviruses, grouping with recently published NS5 partial sequences documented from mosquitoes collected in the Iberian Peninsula, and with Quang Binh virus (isolated in Vietnam) as a close relative. No CTFV sequences were found integrated in their host's genome using a range of specific PCR primers designed to the prM/E, NS3, and NS5 region.
我们描述了从葡萄牙采集的 Theileri 库蚊（Culex theileri）中一种昆虫特异性黄病毒（ISF）的完整遗传特征。这代表了从葡萄牙蚊子中首次分离和全面表征 ISF。该病毒被命名为 CTFV，代表 Theileri 库蚊黄病毒，在 C6/36 Stegomyia albopicta（=Aedes albopictus）细胞系中分离出来，但与其他 ISF 一样，无法在常见的脊椎动物（Vero）细胞中复制。CTFV 基因组编码一个带有 3357 个残基的单一多蛋白，具有所有预期的黄病毒特征。基于迄今为止所有可用的 ISF 序列进行的系统发育分析将 CTFV 置于与库蚊相关的黄病毒中，与最近从伊比利亚半岛采集的蚊子中记录的已发表的 NS5 部分序列一起分组，并与广宁病毒（在越南分离）密切相关。使用针对 prM/E、NS3 和 NS5 区域设计的一系列特定 PCR 引物，在其宿主基因组中未发现 CTFV 序列。
