细菌 III 型分泌系统作为一种蛋白质输送工具，在广泛的生物医学应用中具有重要作用。
Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications.
机构信息
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo 157-8535, Japan.
出版信息
Biotechnol Adv. 2018 Mar-Apr;36(2):482-493. doi: 10.1016/j.biotechadv.2018.01.016. Epub 2018 Feb 2.
Abstract
A protein delivery tool based on bacterial type III secretion system (T3SS) has been broadly applied in biomedical researches. In this review, we summarize various applications of the T3SS-mediate protein delivery which enables translocation of proteins directly into mammalian cells without protein purification. Some of the remarkable advancements include delivery of antigens for therapeutic vaccines, nucleases for genome editing, transcription factors for cellular reprogramming and stem cells differentiation, and signaling molecules for post-translational proteomics studies. With continued improvement of the T3SS-mediated protein delivery tools, even wider application of the technology is anticipated.
摘要
基于细菌 III 型分泌系统（T3SS）的蛋白质递送工具在生物医学研究中得到了广泛应用。在这篇综述中，我们总结了 T3SS 介导的蛋白质递送的各种应用，该方法无需蛋白质纯化即可将蛋白质直接递送到哺乳动物细胞中。一些显著的进展包括递送抗原用于治疗性疫苗、核酸酶用于基因组编辑、转录因子用于细胞重编程和干细胞分化，以及信号分子用于翻译后蛋白质组学研究。随着 T3SS 介导的蛋白质递送工具的不断改进，预计该技术的应用范围将更加广泛。
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