Lindner Florian, Grossmann Sebastian, Diepold Andreas
Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany.
Methods Mol Biol. 2025;2840:115-131. doi: 10.1007/978-1-0716-4047-0_9.
Gram-negative bacteria can use the type III secretion system (T3SS) to inject effector proteins into eukaryotic target cells. In this chapter, we describe the application of a light-controlled T3SS, based on the targeted sequestration of an essential dynamic T3SS component with the help of optogenetic interaction switches. This method enables to control the secretion or injection into eukaryotic cells for a wide range of protein cargos with high temporal and spatial precision.
革兰氏阴性菌可利用III型分泌系统（T3SS）将效应蛋白注入真核靶细胞。在本章中，我们描述了一种光控T3SS的应用，该系统基于借助光遗传学相互作用开关对一种必需的动态T3SS组分进行靶向隔离。这种方法能够以高时空精度控制多种蛋白质货物向真核细胞的分泌或注射。
