Laboratory of Molecular Oncobiology, Institute of Gene Biology RAS, 119334 Moscow, Russia.
Institute for Environmental Science and Biotechnology, Derzhavin Tambov State University, 392020 Tambov, Russia.
Int J Mol Sci. 2023 Feb 1;24(3):2783. doi: 10.3390/ijms24032783.
Due to their chemical, mechanical, and optical properties, 2D ultrathin nanomaterials have significant potential in biomedicine. However, the cytotoxicity of such materials, including their mutual increase or decrease, is still not well understood. We studied the effects that graphene oxide (GO) nanolayers (with dimensions 0.1-3 μm and average individual flake thickness less than 1 nm) and ZrS nanoribbons (length more than 10 μm, width 0.4-3 μm, and thickness 50-120 nm) have on the viability, cell cycle, and cell death of HCT116 colon carcinoma cells. We found that ZrS exhibited strong cytotoxicity by causing apoptotic cell death, which was in contrast to GO. When adding GO to ZrS, ZrS was significantly less toxic, which may be because GO inhibits the effects of cytotoxic hydrogen sulfide produced by ZrS. Thus, using zirconium trisulfide nanoribbons as an example, we have demonstrated the ability of graphene oxide to reduce the cytotoxicity of another nanomaterial, which may be of practical importance in biomedicine, including the development of biocompatible nanocoatings for scaffolds, theranostic nanostructures, and others.
二维超薄纳米材料由于其化学、机械和光学特性，在生物医学领域具有重要的应用潜力。然而，这类材料的细胞毒性，包括它们之间的相互增加或减少，仍未被很好地理解。我们研究了氧化石墨烯（GO）纳米层（尺寸为 0.1-3μm，平均单层厚度小于 1nm）和 ZrS 纳米带（长度大于 10μm，宽度 0.4-3μm，厚度 50-120nm）对 HCT116 结肠癌细胞活力、细胞周期和细胞死亡的影响。我们发现 ZrS 通过诱导细胞凋亡而表现出很强的细胞毒性，这与 GO 相反。当向 ZrS 中添加 GO 时，ZrS 的毒性显著降低，这可能是因为 GO 抑制了 ZrS 产生的细胞毒性氢硫化物的作用。因此，我们以三硫化二锆纳米带为例，证明了氧化石墨烯能够降低另一种纳米材料的细胞毒性，这在生物医学领域可能具有实际意义，包括开发用于支架的生物相容性纳米涂层、治疗诊断纳米结构等。
