Inhibition of biofilm formation by extracts of sp. 20J, a bacterium with wide-spectrum quorum quenching activity.

: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by , a pathogen involved in tooth decay. : To obtain inhibition of biofilm formation by by extracts obtained from the marine bacterium sp. 20J interfering with the AI-2 quorum sensing system. : The AI-2 inhibitory activity was tested with the biosensors BB170 and JMH597. ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method. : The addition of the cell extract from sp. 20J reduced biofilm formation in ATCC25175 by 40-50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in DSM20627 and 7747 biofilms. : The ability of sp. 20J to interfere with AI-2 and inhibit biofilm formation in was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.