Zhang Xiaoning, Gao Pei, Hollimon Valerie, Brodus DaShan, Johnson Arion, Hu Hongmei
College of Biotechnology, Southwest University, Chongqing, 400715, China.
Department of Chemistry, Eastern Kentucky University, 521 Lancaster Ave, Richmond, KY, 40475, USA.
Chem Cent J. 2018 Feb 7;12(1):10. doi: 10.1186/s13065-018-0385-6.
Thiol groups grafted silicon surface was prepared as previously described. 1H,1H,2H,2H-perfluorodecanethiol (PFDT) molecules were then immobilized on such a surface through disulfide bonds formation. To investigate the contribution of PFDT coating to antifouling, the adhesion behaviors of Botryococcus braunii (B. braunii) and Escherichia coli (E. coli) were studied through biofouling assays in the laboratory. The representative microscope images suggest reduced B. braunii and E. coli accumulation densities on PFDT integrated silicon substrate. However, the antifouling performance of PFDT integrated silicon substrate decreased over time. By incubating the aged substrate in 10 mM TCEP·HCl solution for 1 h, the fouled PFDT coating could be removed as the disulfide bonds were cleaved, resulting in reduced absorption of algal cells and exposure of non-fouled silicon substrate surface. Our results indicate that the thiol-terminated substrate can be potentially useful for restoring the fouled surface, as well as maximizing the effective usage of the substrate.
如前所述制备了接枝硫醇基团的硅表面。然后通过形成二硫键将1H,1H,2H,2H-全氟癸硫醇（PFDT）分子固定在该表面上。为了研究PFDT涂层对防污的贡献，通过实验室中的生物污垢测定法研究了布朗葡萄藻（B. braunii）和大肠杆菌（E. coli）的粘附行为。代表性的显微镜图像表明，在集成PFDT的硅基板上，布朗葡萄藻和大肠杆菌的积累密度降低。然而，集成PFDT的硅基板的防污性能会随时间下降。通过将老化的基板在10 mM TCEP·HCl溶液中孵育1小时，随着二硫键的断裂，被污染的PFDT涂层可以被去除，从而减少藻类细胞的吸附并暴露未被污染的硅基板表面。我们的结果表明，硫醇封端的基板可能有助于恢复被污染的表面，并最大限度地提高基板的有效利用率。
