Felmingham D, O'Hare M D, Robbins M J, Wall R A, Williams A H, Cremer A W, Ridgway G L, Grüneberg R N
Drugs Exp Clin Res. 1985;11(5):317-29.
The minimal inhibitory concentrations (MICs) of nalidixic acid, pipemidic acid, cinoxacin, oxolinic acid, flumequine, pefloxacin, acrosoxacin, amifloxacin, norfloxacin, enoxacin, ofloxacin and ciprofloxacin were determined for a range of clinical isolates. MICs were determined using an agar dilution technique in Mueller-Hinton agar supplemented with 10% lysed horse blood. The inoculum used was approximately 10(4) colony forming units, contained in 10 microliters Mueller-Hinton broth, which was applied to the agar plates using a multipoint inoculator. Following inoculation, plates were incubated in conditions appropriate for the organisms under investigation. The MIC of each antimicrobial for each isolate examined was determined as the lowest concentration of the antimicrobial which completely inhibited growth of the inoculum. The minimum concentrations required to inhibit the growth of 50% (MIC50) and 90% (MIC90) of the organisms examined were also determined. All of the more recently synthesised 4-quinolones showed considerably greater activity than the parent compounds, nalidixic acid, pipemidic acid and cinoxacin, against the range of organisms used in this study. Ciprofloxacin and ofloxacin were the two most active of the 4-quinolones examined.
测定了一系列临床分离株对萘啶酸、吡哌酸、西诺沙星、恶喹酸、氟甲喹、培氟沙星、阿克罗沙星、阿米氟沙星、诺氟沙星、依诺沙星、氧氟沙星和环丙沙星的最低抑菌浓度（MIC）。MIC采用琼脂稀释法在补充有10%溶血马血的穆勒-欣顿琼脂中测定。使用的接种物约为10⁴ 个菌落形成单位，包含在10微升穆勒-欣顿肉汤中，用多点接种器将其接种到琼脂平板上。接种后，将平板在适合所研究微生物的条件下培养。所检测的每种抗菌药物对每个分离株的MIC被确定为完全抑制接种物生长的抗菌药物的最低浓度。还测定了抑制所检测微生物50%（MIC50）和90%（MIC90）生长所需的最低浓度。在本研究中使用的一系列微生物中，所有最近合成的4-喹诺酮类药物均显示出比母体化合物萘啶酸、吡哌酸和西诺沙星显著更高的活性。环丙沙星和氧氟沙星是所检测的4-喹诺酮类药物中活性最强的两种。
