Key Lab of Special Display Technology, Ministry of Education, National Engineering Lab of Special Display Technology, State Key Lab of Advanced Display Technology, Academy of Opto-Electronic Technology, Hefei University of Technology, Hefei 230009, China.
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Biosens Bioelectron. 2018 May 15;105:121-128. doi: 10.1016/j.bios.2018.01.035. Epub 2018 Feb 2.
Chiral recognition of α-amino acids is attracting increasing interest due to the importance of α-amino acids in protein metabolism as well as in food products and pharmaceuticals. Organic electrochemical transistors (OECTs) with gate electrodes modified with molecularly imprinted polymer (MIP) films were fabricated and successfully used as highly selective and sensitive chiral recognition biosensors for d/l-tryptophan (d/l-Trp) and d/l-tyrosine (d/l-Tyr). The MIP films, which can specifically recognize and has an electrocatalytic effect on the oxidation of Trp and Tyr, together with the amplification function of an OECT, provide a highly sensitive and selective OECT biosensor. The sensor showed a linear response range for l-Trp and L-Tyr from 300 nM to 10 μM with a sensitivity of 3.19 and 3.64 μA/μM, respectivity. And the detection limit for L-Trp and L-Tyr is of 2 nM and 30 nM (S/N > 3). The selectivity factors of L-Trp, D-Trp, L-Tyr and D-Tyr to their enantiomers are 11.6, 3.5, 14.5 and 2.6, respectively. This method can pave the way for widespread applications of OECT-based sensors in chiral material identification.
由于 α-氨基酸在蛋白质代谢以及食品和药物中具有重要性，因此对其对映体的手性识别引起了越来越多的关注。制备了带有分子印迹聚合物（MIP）薄膜的栅极电极修饰的有机电化学晶体管（OECT），并成功地用作 d/l-色氨酸（d/l-Trp）和 d/l-酪氨酸（d/l-Tyr）的高选择性和灵敏的手性识别生物传感器。MIP 薄膜可以特异性地识别并对 Trp 和 Tyr 的氧化具有电催化作用，再加上 OECT 的放大功能，为高度灵敏和选择性的 OECT 生物传感器提供了可能。该传感器对 l-Trp 和 L-Tyr 的线性响应范围为 300 nM 至 10 μM，灵敏度分别为 3.19 和 3.64 μA/μM。并且 L-Trp 和 L-Tyr 的检测限分别为 2 nM 和 30 nM（S/N > 3）。L-Trp、D-Trp、L-Tyr 和 D-Tyr 对其对映体的选择性因子分别为 11.6、3.5、14.5 和 2.6。该方法为基于 OECT 的传感器在手性材料识别中的广泛应用铺平了道路。
