Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
Ann Allergy Asthma Immunol. 2018 Feb;120(2):155-163. doi: 10.1016/j.anai.2017.12.005.
An allergic rhinitis (AR) diagnosis is based on typical nasal symptoms and allergen sensitization testing. However, it is sometimes difficult to distinguish AR from non-AR, and it is especially difficult to identify the causal allergen for immunotherapy of patients with AR.
To identify differences among patients with AR, subjects with asymptomatic sensitization (AS), and subjects without sensitization (NS) that could facilitate the diagnosis of AR.
We used RNA sequencing to examine differential gene expression in unstimulated and allergen-stimulated peripheral basophils from participants with NS, AS, and AR. We selected genes that were upregulated after allergen stimulation and showed differences in expression in patients with AR compared with subjects with AS and NS. Basophil surface expression of 1 gene product was examined by flow cytometry. The usefulness of gene expression in the diagnosis of AR was examined with receiver operating characteristic curves.
Expression of cytokine receptor-like factor 2 and its product, thymic stromal lymphopoietin receptor (TSLPR), was significantly increased in basophils of patients with AR after allergen stimulation. A significantly larger percentage of TSLPR-positive cells was observed after allergen-specific stimulation of basophils from patients with AR compared with subjects with AS. Basophil TSLPR expression was as good as or better than CD203c expression in discriminating between patients with AR and subjects with AS, as judged by receiver operating characteristic curves.
Our data suggest that TSLPR expression on basophils was specifically upregulated by allergen stimulation and might be useful for the identification of the causative allergen in patients with AR.
变应性鼻炎（AR）的诊断基于典型的鼻部症状和过敏原致敏检测。然而，有时很难将 AR 与非 AR 区分开来，对于需要进行 AR 免疫治疗的患者，尤其难以确定其致病过敏原。
确定 AR 患者、无症状致敏（AS）患者和无致敏（NS）患者之间的差异，以帮助 AR 的诊断。
我们使用 RNA 测序来检测 NS、AS 和 AR 患者外周血嗜碱性粒细胞在未刺激和过敏原刺激后的差异基因表达。我们选择了在过敏原刺激后上调且在 AR 患者中与 AS 和 NS 患者相比表达存在差异的基因。通过流式细胞术检测 1 个基因产物的嗜碱性粒细胞表面表达。通过受试者工作特征曲线来评估基因表达在 AR 诊断中的作用。
在过敏原刺激后，AR 患者的嗜碱性粒细胞中细胞因子受体样因子 2（cytokine receptor-like factor 2，CRLF2）及其产物胸腺基质淋巴细胞生成素受体（thymic stromal lymphopoietin receptor，TSLPR）的表达显著增加。与 AS 患者相比，AR 患者的嗜碱性粒细胞在经过过敏原特异性刺激后，TSLPR 阳性细胞的比例明显更大。通过受试者工作特征曲线判断，嗜碱性粒细胞 TSLPR 表达在区分 AR 患者和 AS 患者方面与 CD203c 表达一样好，甚至更好。
我们的数据表明，过敏原刺激特异性地上调了嗜碱性粒细胞上的 TSLPR 表达，可能有助于鉴定 AR 患者的致病过敏原。
