Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, Napoli, Italy; Centro di Ricerca Interdipartimentale sui Biomateriali (CRIB), Università di Napoli Federico II, P.le Tecchio 80, Napoli, Italy; Dipartimento di Chimica, Materiali e Produzione Industriale (DICMAPI), Università di Napoli Federico II, P.le Tecchio 80, Napoli, Italy.
Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, Napoli, Italy.
Colloids Surf B Biointerfaces. 2018 Apr 1;164:232-239. doi: 10.1016/j.colsurfb.2018.01.028. Epub 2018 Jan 31.
The development of innovative nano-bio-encapsulation systems continues to be an area of intense activity as the demand of improved delivery systems is constantly increasing in several fields including nanomedicine. For this purpose, an important goal is carrying out appropriate engineering of the surface of these nanocarriers to satisfy the organ target features for an effective in situ release and elucidate the mechanism of action which most of the time is neglected. Here, an oil-in-water (O/W) nanoemulsion coated with a polysaccharide layer film - i.e. a glycol chitosan modified with a thiol moiety - was used as nanocarrier to convey a promising poorly water-soluble nature based drug, curcumin. The final nanocarrier was completely bio-compatible and bio-stable. We investigated the enhancement of the effect of curcumin loaded in our system across monolayers of intestinal epithelial cells CaCo-2 in Transwell culture. Such in vitro platform resulted suitable to evaluate the functionality of the proposed nanocarrier and its adhesion towards the mucosal epithelial layer and, as applicative example, to investigate the anti-inflammatory effects exerted by the encapsulation of curcumin.
作为对改进输送系统的需求在包括纳米医学在内的多个领域不断增加，创新的纳米生物包封系统的发展仍然是一个活跃的研究领域。为此，一个重要的目标是对这些纳米载体的表面进行适当的工程设计，以满足有机靶标特征，实现有效原位释放，并阐明作用机制，而这一机制在大多数情况下被忽视。在这里，我们使用一种油包水（O/W）纳米乳液作为纳米载体，该乳液涂有多糖层膜，即带有巯基部分的乙二醇壳聚糖，来传递一种有前途的水溶性差的天然药物姜黄素。最终的纳米载体完全具有生物相容性和生物稳定性。我们研究了在 Transwell 培养的肠上皮细胞 CaCo-2单层中负载姜黄素的系统的效果增强。这种体外平台适合评估所提出的纳米载体的功能及其对粘膜上皮层的黏附性，并可作为应用实例，研究姜黄素包封所产生的抗炎作用。
