Mazzoleni Sofia, Rovatsos Michail, Schillaci Odessa, Dumas Francesca
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, via Archirafi 18.
Faculty of Science, Department of Ecology, Viničná 7, Charles University, Pragha 2, Czech Republic.
Comp Cytogenet. 2018 Jan 24;12(1):27-40. doi: 10.3897/CompCytogen.v12i1.19381. eCollection 2018.
We explored the topology of 18S and 28S rDNA units by fluorescence hybridization (FISH) in the karyotypes of thirteen species representatives from major groups of Primates and (Günther, 1876) (Scandentia), in order to expand our knowledge of Primate genome reshuffling and to identify the possible dispersion mechanisms of rDNA sequences. We documented that rDNA probe signals were identified on one to six pairs of chromosomes, both acrocentric and metacentric ones. In addition, we examined the potential homology of chromosomes bearing rDNA genes across different species and in a wide phylogenetic perspective, based on the DAPI-inverted pattern and their synteny to human. Our analysis revealed an extensive variability in the topology of the rDNA signals across studied species. In some cases, closely related species show signals on homologous chromosomes, thus representing synapomorphies, while in other cases, signal was detected on distinct chromosomes, leading to species specific patterns. These results led us to support the hypothesis that different mechanisms are responsible for the distribution of the ribosomal DNA cluster in Primates.
我们通过荧光杂交（FISH）技术，在灵长目主要类群的13个物种代表以及树鼩（Günther，1876）的核型中探索了18S和28S rDNA单元的拓扑结构，以拓展我们对灵长类基因组重排的认识，并确定rDNA序列可能的分散机制。我们记录到rDNA探针信号在一到六对染色体上被识别，包括近端着丝粒染色体和中着丝粒染色体。此外，我们基于DAPI反转模式及其与人类的同线性，从广泛的系统发育角度研究了不同物种中携带rDNA基因的染色体的潜在同源性。我们的分析揭示了在所研究物种中rDNA信号拓扑结构的广泛变异性。在某些情况下，亲缘关系密切的物种在同源染色体上显示信号，从而代表了共有衍征，而在其他情况下，信号在不同的染色体上被检测到，导致物种特异性模式。这些结果使我们支持这样一种假说，即不同的机制负责灵长类动物中核糖体DNA簇的分布。
