Ji Wenxin, Qiu Chunli, Wang Mao, Mao Ning, Wu Shaofeng, Dai Yinhai
Department of Surgical Oncology, Shaanxi University of Chinese Medicine, Xi'an 710032, PR China.
Xianyang Central Hospital, Xianyang 712000, PR China.
Biochem Biophys Res Commun. 2018 Feb 26;497(1):122-126. doi: 10.1016/j.bbrc.2018.02.036. Epub 2018 Feb 6.
The circRNAs are differentially expressed in a wide range of cancers in regulating their initiation and progression, and could be used to make a diagnosis for some diseases like tumor as a new biomarker. However, the correlation and the mechanism of action between circRNAs and colorectal cancer (CRC) are still unclear. In this study, by using qRT-PCRs, we detected the expression level of hsa_circ_0001649 in tissue and serum samples from CRC patients, and the cultured cell has been detected. We found that the hsa_circ_0001649 in CRC is significantly lower than the expression level of correspondent nontumorous tissues (n = 64, P < 0.01). We also tested the HCT116 cell lines, and the similar result is observed (n = 15, P < 0.01). Moreover, we detected the serum samples obtained before and after surgery, showing significantly the expression level of hsa_circ_0001649 in the same patient is up-regulated after surgery (n = 18, P < 0.01). Besides, we analyzed the correlation between clinicopathological date and the expression level of hsa_circ_0001649, we found that hsa_circ_0001649 expression level is closely associated with pathological differentiation (P = 0.037), and the result also illustrated that the expression level of hsa_circ_0001649 is no direct correlation with age, gender, TMN stage, lymphatic metastasis, CEA, CA19-9, and CA-724 levels. The area under the receiver operating characteristic (ROC) curve was 0.857. In conclusion, this study showed that the expression level of hsa_circ_0001649 was down-regulated in CRC and could use it as a new biomarker for specific and sensitive inspection of CRC.
环状RNA（circRNAs）在多种癌症的发生和发展过程中存在差异表达，可作为一种新型生物标志物用于某些疾病如肿瘤的诊断。然而，circRNAs与结直肠癌（CRC）之间的相关性及作用机制仍不清楚。在本研究中，我们通过定量逆转录聚合酶链反应（qRT-PCR）检测了CRC患者组织和血清样本中hsa_circ_0001649的表达水平，并对培养细胞进行了检测。我们发现，CRC中hsa_circ_0001649的表达水平显著低于相应的非肿瘤组织（n = 64，P < 0.01）。我们还检测了HCT116细胞系，观察到类似结果（n = 15，P < 0.01）。此外，我们检测了手术前后获取的血清样本，结果显示同一患者术后hsa_circ_0001649的表达水平显著上调（n = 18，P < 0.01）。此外，我们分析了临床病理数据与hsa_circ_0001649表达水平之间的相关性，发现hsa_circ_0001649表达水平与病理分化密切相关（P = 0.037），结果还表明hsa_circ_0001649表达水平与年龄、性别、TMN分期、淋巴转移、癌胚抗原（CEA）、糖类抗原19-9（CA19-9）和糖类抗原724（CA-724）水平无直接相关性。受试者工作特征（ROC）曲线下面积为0.857。总之，本研究表明hsa_circ_0001649在CRC中表达下调，可作为CRC特异性和敏感性检测的新型生物标志物。
