Yang Ning, Xu Bin, Kong Peng, Han Mei, Li Bing-Hui
The Second Department of General Surgery, Fourth Hospital of Hebei Medical University.
Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, Hebei, P.R. China.
Medicine (Baltimore). 2020 Jul 10;99(28):e21224. doi: 10.1097/MD.0000000000021224.
A great many circular RNAs (circRNAs) exist in different types of mammalian cells. Previous studies have verified that a low level of hsa_circ_0002320 is present in gastric cancer and that it might represent a good prognostic indicator. However, its value in colorectal cancer (CRC) is unclear. The aim of this research was to explore the value of hsa_circ_0002320 as a potential diagnostic biomarker for CRC prognosis.Plasma samples, CRC tissues, and adjacent normal tissues were obtained from 50 patients with CRC, before any treatment, and 100 plasma samples were acquired from healthy individuals. Hsa_circ_0002320 levels in these samples were analyzed by reverse transcription-quantitative polymerase chain reaction. Correlations between hsa_circ_0002320, clinicopathological characteristics, and overall survival (OS) of CRC patients were also investigated. Receiver-operating characteristic (ROC) curve analysis was used to assess the value of hsa_circ_0002320 for CRC diagnosis. Finally, a bioinformatics analysis was performed to verify the effect of hsa_circ_0002320 on CRC prognosis.Expression levels of hsa_circ_0002320 were significantly decreased in CRC plasma (P < .05). The expression level of hsa_circ_0002320 was significantly correlated with OS time (P < .05). Higher hsa_circ_0002320 reflected significantly greater OS; the HR of high hsa_circ_0002320 was 0.161 (95% CI, 0.066-0.393; P = .000). The area under the ROC curve of hsa_circ_0002320 in CRC was 0.823, which was higher than for the carcinoembryogenic antigen (area under the curve = 0.764). Kaplan-Meier analysis showed that CRC patients with low expression of hsa_circ_0002320 exhibited poorer OS times than those with high expression.Hsa_circ_0002320 could be a novel, noninvasive diagnostic blood biomarker for CRC prognosis.
许多环状RNA（circRNA）存在于不同类型的哺乳动物细胞中。先前的研究已证实，胃癌中存在低水平的hsa_circ_0002320，它可能是一个良好的预后指标。然而，其在结直肠癌（CRC）中的价值尚不清楚。本研究的目的是探讨hsa_circ_0002320作为CRC预后潜在诊断生物标志物的价值。
在50例未经任何治疗的CRC患者中获取血浆样本、CRC组织和癌旁正常组织，并从健康个体中采集100份血浆样本。通过逆转录定量聚合酶链反应分析这些样本中hsa_circ_0002320的水平。还研究了hsa_circ_0002320、临床病理特征与CRC患者总生存期（OS）之间的相关性。采用受试者工作特征（ROC）曲线分析评估hsa_circ_0002320对CRC诊断的价值。最后，进行生物信息学分析以验证hsa_circ_0002320对CRC预后的影响。
CRC血浆中hsa_circ_0002320的表达水平显著降低（P < 0.05）。hsa_circ_0002320的表达水平与OS时间显著相关（P < 0.05）。较高的hsa_circ_0002320反映出显著更长的OS；高hsa_circ_0002320的HR为0.161（95%CI，0.066 - 0.393；P = 0.000）。hsa_circ_0002320在CRC中的ROC曲线下面积为0.823，高于癌胚抗原（曲线下面积 = 0.764）。Kaplan - Meier分析显示，hsa_circ_0002320低表达的CRC患者的OS时间比高表达患者更差。
hsa_circ_0002320可能是一种用于CRC预后的新型非侵入性诊断血液生物标志物。
