Wei Yalan, Huang Ling, Cao Jinghui, Wang Chenghui, Yan Jizhou
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Department of Developmental Biology, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, China.
Front Physiol. 2018 Jan 25;9:8. doi: 10.3389/fphys.2018.00008. eCollection 2018.
Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding Flk1-transgenic larvae model to examine the predatory fish's histology in multiple tissues, and the global gene expression profile in the liver. 180 days of feeding trial show that there are no significantly morphological changes in intestine, liver, kidney, and sex gonads between fish fed with Flk1 transgenic fish diet (TFD) and fish fed with regular food meal (RFM). However, a characteristic skin spot and autofluorescence increase in the theca of follicle are observed in F1 generation of TFD fish. Liver RNA-sequencing analyses demonstrate that 53 out of 56712 genes or isoforms are differentially transcribed, and mostly involved in proteolysis in extracellular region. According to GO enrichment terms these deregulated genes function in catalytic activity, steroid storing, lipid metabolic process and N-Glycan biosynthesis. These results suggest that a long term of Flk1-transgenic fish diet could alter certain metabolic pathways and possibly cause related tissue deformation. Compared to the previous reports, our feasible transgenic dietary assess system could evaluate subchronic and potential health impact of transgenic fish diet by combining multi-tissue histology and liver transcriptome analyses.
基因工程，也称为基因修饰，正面临着水产养殖和水产品日益增长的需求。尽管已经培育出了各种转基因水产，但在转基因生物进入我们的食物链之前，评估其对人类健康的生物安全性非常重要。为此，我们建立了一个斑马鱼野生成体喂养Flk1转基因幼虫的模型，以检查捕食性鱼类多个组织的组织学以及肝脏中的全局基因表达谱。180天的喂养试验表明，喂食Flk1转基因鱼饲料（TFD）的鱼和喂食常规食物餐（RFM）的鱼在肠道、肝脏、肾脏和性腺方面没有明显的形态变化。然而，在TFD鱼的F1代中观察到了特征性的皮肤斑点以及卵泡膜中自发荧光增加。肝脏RNA测序分析表明，56712个基因或异构体中有53个转录存在差异，且大多参与细胞外区域的蛋白水解。根据基因本体论（GO）富集术语，这些失调的基因在催化活性、类固醇储存、脂质代谢过程和N-聚糖生物合成中发挥作用。这些结果表明，长期食用Flk1转基因鱼饲料可能会改变某些代谢途径，并可能导致相关组织变形。与之前的报道相比，我们可行的转基因饮食评估系统可以通过结合多组织组织学和肝脏转录组分析来评估转基因鱼饲料的亚慢性和潜在健康影响。
