Ogawa H, Tsunematsu T
Clin Exp Immunol. 1986 Apr;64(1):88-93.
We studied the in vivo effect of transfer of nonspecific suppressor spleen cells into syngeneic Lewis rats. The rats were immunized with Freund's adjuvant on day 0, and then given 5 X 10(7) cells on days--7, 0 and 7. These cells had been incubated for 3 days, with or without mitogenic stimulation. Transfer of the cultured cells markedly diminished the severity of adjuvant arthritis (AA). On the contrary, transfer of concanavalin A (Con A)-stimulated cells led to no suppressive activity. These results differed from findings in a prior in vitro experiment, in which the suppressive influence of previously cultured cells on T and B lymphocytes proliferation rates was examined and significant suppressive activity was detected in Con A-stimulated cells but not in cultured cells. Suppressor activity by transfer of solely cultured cells was identified in T cell fractions. Transfer of 5 X 10(7) spleen cells proved to be the optimal dose for suppressing AA, and transfer of less than 2 X 10(7) cells had no significant effect. The number and time of transfer were also important factors in the suppression. Each group of transfer on days--7, 0 and 7, days--7 and 0, and days 0 and 7 led to a similar reduction in the severity of AA, and was less prominent in the group injected on days 7 and 14. A single injection on days--7, 0, or 7 revealed minimal or no effects. These observations indicate that the transfer of in vitro cultured spleen cells nonspecifically modified the course of rat AA in vivo, thereby differing from the results of the suppressor activity seen in vitro. The transfer of cultured cells, as a potential tool for the treatment of clinical diseases warrants further attention.
我们研究了将非特异性抑制性脾细胞转移至同基因Lewis大鼠体内的效应。在第0天用弗氏佐剂对大鼠进行免疫，然后在第 -7、0和7天给予5×10⁷个细胞。这些细胞已培养3天，有无丝裂原刺激。转移培养细胞显著减轻了佐剂性关节炎（AA）的严重程度。相反，转移伴刀豆球蛋白A（Con A）刺激的细胞则无抑制活性。这些结果与之前的体外实验结果不同，在体外实验中检测了先前培养的细胞对T和B淋巴细胞增殖率的抑制影响，发现Con A刺激的细胞有显著抑制活性，而培养细胞则没有。在T细胞组分中鉴定出仅通过转移培养细胞产生的抑制活性。转移5×10⁷个脾细胞被证明是抑制AA的最佳剂量，转移少于2×10⁷个细胞则无显著效果。转移的数量和时间也是抑制中的重要因素。在第 -7、0和7天、第 -7和0天以及第0和7天进行的每组转移都导致AA严重程度有类似程度的降低，而在第7和14天注射的组中这种降低不太明显。在第 -7、0或7天单次注射显示效果极小或无效果。这些观察结果表明，体外培养的脾细胞转移在体内非特异性地改变了大鼠AA的病程，从而与体外所见的抑制活性结果不同。作为治疗临床疾病的潜在工具，培养细胞的转移值得进一步关注。
