Department of Biochemistry, University of Utah, Salt Lake City, UT, 84112, USA.
Anal Bioanal Chem. 2018 Mar;410(8):2053-2057. doi: 10.1007/s00216-018-0899-8. Epub 2018 Feb 9.
The 2017 Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson for "developing cryo-electron microscopy (cryo-EM) for the high-resolution structure determination of biomolecules in solution." This feature article summarizes some of the major achievements leading to the development of cryo-EM and recent technological breakthroughs that have transformed the method into a mainstream tool for structure determination.
2017 年诺贝尔化学奖授予雅克·杜博歇（Jacques Dubochet）、约阿希姆·弗兰克（Joachim Frank）和理查德·亨德森（Richard Henderson），以表彰他们“发展了用于溶液中生物分子高分辨率结构测定的冷冻电子显微镜（cryo-EM）”。本文综述了促成 cryo-EM 发展的一些主要成就，以及最近的技术突破，这些突破使该方法转变为结构测定的主流工具。
