义齿性口炎患者细胞因子基因多态性的临床研究。

Cytokine gene polymorphism in denture stomatitis patients: A clinical study.

机构信息

Department of Prosthodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.

Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

出版信息

Oral Dis. 2018 Sep;24(6):983-992. doi: 10.1111/odi.12844. Epub 2018 Jun 11.

DOI:10.1111/odi.12844

PMID:29423993

Abstract

OBJECTIVE

This clinical study investigated the association between cytokine gene polymorphism and Candida growth in denture stomatitis (DS) patients.

SUBJECTS AND METHODS

Saliva and blood samples of 160 complete denture wearers (80 healthy controls and 80 with DS) were collected for mycological and gene polymorphism testing, respectively. Salivary Candida growth and TNF-α, TGF-β, IL-6, and IL-10 genotypes were investigated. Data were analyzed using Student's t test, Mann-Whitney U test, chi-square analysis, and continuity (yates) correction tests (p < .05).

RESULTS

Candida albicans colony counts in saliva were significantly higher in the DS group and in the TNF-α GG genotype (p < .05). TGF-β TC GG and TGF-β CC GG haplotypes were significantly higher in DS and control groups, respectively (p < .05). C. albicans colony counts were significantly higher in control group in the TGF-β TC GG haplotype (p < .05). Candida glabrata colony counts were significantly higher in the DS group than the control group in IL-6 GG genotype (p < .05). The difference between DS types in IL-6 genotypes was significant with lower expression level in DS type 3 than DS type 1 and also type 2 (p ≤ .01).

CONCLUSION

The significant differences in some genotypes of the TNF-α, TGF-β, and IL-6 in DS patients are promising in understanding the host defense in DS.

摘要

目的

本临床研究旨在探讨细胞因子基因多态性与义齿性口炎（DS）患者白色念珠菌生长的关系。

对象和方法

分别采集 160 名全口义齿佩戴者（80 名健康对照者和 80 名 DS 患者）的唾液和血液样本，进行微生物学和基因多态性检测。检测唾液中白色念珠菌生长情况及 TNF-α、TGF-β、IL-6 和 IL-10 基因型。采用 Student's t 检验、Mann-Whitney U 检验、卡方检验和连续性（yates）校正检验（p<0.05）对数据进行分析。

结果

DS 组和 TNF-α GG 基因型患者的唾液中白色念珠菌菌落计数明显较高（p<0.05）。TGF-β TC GG 和 TGF-β CC GG 单倍型在 DS 组和对照组中分别明显较高（p<0.05）。在 TGF-β TC GG 单倍型中，对照组的白色念珠菌菌落计数明显较高（p<0.05）。与对照组相比，IL-6 GG 基因型的 DS 组白色念珠菌和光滑念珠菌菌落计数明显较高（p<0.05）。在 DS 类型中，IL-6 基因型的差异具有统计学意义，DS 类型 3 与 DS 类型 1 和类型 2 相比，表达水平较低（p≤0.01）。