Pinto Telma Maria Silva, Neves Ana Christina Claro, Leão Mariella Vieira Pereira, Jorge Antonio Olavo Cardoso
Department of Dentistry, University of Taubaté, Taubaté, SP, Brazil.
J Appl Oral Sci. 2008 Nov-Dec;16(6):385-90. doi: 10.1590/s1678-77572008000600006.
The use of denture is known to increase the carriage of Candida in healthy patients, and the proliferation of Candida albicans strains can be associated with denture-induced stomatitis. The aim of this study was to evaluate the use of vinegar as an antimicrobial agent for control of Candida spp. in complete upper denture wearers. Fifty-five patients were submitted to a detailed clinical interview and oral clinical examination, and were instructed to keep their dentures immersed in a 10% vinegar solution (pH less than 3) overnight for 45 days. Before and after the experimental period, saliva samples were collected for detection of Candida, counting of cfu/mL and identification of species by phenotypical tests (germ tube formation, chlamidoconidia production, and carbohydrate fermentation and assimilation). The results were analyzed using Spearman's correlation and Student's t-test (p<or=0.05). Candida yeasts were present in 87.3% of saliva samples before the treatment. A significant reduction was verified in CFU/mL counts of Candida after treatment. A positive correlation between Candida and denture stomatitis was verified, since the decrease of cfu/mL counts was correlated with a reduction in cases of denture stomatitis. Although it was not able to eliminate C. albicans, the immersion of the complete denture in 10% vinegar solution, during the night, reduced the amounts (cfu/mL) of Candida spp. in the saliva and the presence of denture stomatitis in the studied patients.
众所周知,佩戴假牙会增加健康患者口腔念珠菌的携带量,白色念珠菌菌株的增殖可能与假牙性口炎有关。本研究的目的是评估醋作为一种抗菌剂,用于控制全口上假牙佩戴者口腔念珠菌的效果。55名患者接受了详细的临床访谈和口腔临床检查,并被要求将假牙浸泡在10%的醋溶液(pH值小于3)中过夜,持续45天。在实验期前后,收集唾液样本用于检测念珠菌、计算每毫升菌落形成单位(cfu/mL)并通过表型试验(芽管形成、厚壁孢子产生以及碳水化合物发酵和同化)鉴定菌种。结果采用Spearman相关性分析和Student's t检验进行分析(p≤0.05)。治疗前,87.3%的唾液样本中存在念珠菌酵母。治疗后念珠菌的cfu/mL计数显著降低。念珠菌与假牙性口炎之间存在正相关,因为cfu/mL计数的降低与假牙性口炎病例的减少相关。尽管不能消除白色念珠菌,但夜间将全口假牙浸泡在10%的醋溶液中,可减少所研究患者唾液中念珠菌的数量(cfu/mL)以及假牙性口炎的发生。