用 HPLC-MS/MS 测定红豆杉红色假种皮中的紫杉烷成分。
Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC-MS/MS.
机构信息
Dresden University of Applied Science, Faculty of Agriculture/Environment/Chemistry, Dresden, Germany.
Institute of Legal Medicine, Medical Faculty Carl Gustav Carus, Dresden Technical University, Dresden, Germany.
出版信息
Phytochem Anal. 2018 Sep;29(5):446-451. doi: 10.1002/pca.2748. Epub 2018 Feb 9.
INTRODUCTION
Taxus baccata L. is an evergreen conifer whose plant parts are cardiotoxic. Only the red arils of the berries are described as non-toxic and taxane-free.
OBJECTIVE
Extraction and HPLC-MS/MS methods were developed for the investigation of the Taxus compounds 3,5-dimethoxyphenol, 10-deacetylbaccatin III, baccatin III, cephalomannine, taxol A and taxinine M in the red arils of the yew berries.
METHODOLOGY
A liquid-liquid extraction method for the red arils of the fruits from three yews were developed. An accurate (ESI+) HPLC-MS/MS method was performed for the simultaneous detection and determination of the target compounds in multiple reaction monitoring (MRM) mode.
RESULTS
All Taxus agents obtained were detected in the red arils. Highest concentrations were determined for baccatin III and 10-deacetylbaccatin III.
CONCLUSION
The developed quantitative method is reliable and selective and was successfully applied for quantification of selected Taxus ingredients in red arils of Taxus baccata. It was disproved that the red arils of the berries do not contain the selected Taxus compounds.
简介
欧洲红豆杉是一种常绿针叶树，其植物部分具有心脏毒性。只有红色浆果的假种皮被描述为无毒且不含紫杉醇。
目的
开发提取和 HPLC-MS/MS 方法，以调查红豆杉中的化合物 3,5-二甲氧基苯酚、10-去乙酰基紫杉醇 III、紫杉醇 III、cephalomannine、紫杉醇 A 和 taxinine M 在红豆杉浆果的红色假种皮中的存在。
方法
为三种红豆杉的果实的红色假种皮开发了一种液液提取方法。采用精确（ESI+）HPLC-MS/MS 方法，以多重反应监测 (MRM) 模式同时检测和定量目标化合物。
结果
在所研究的三种红豆杉中，均检测到所有的红豆杉药物。在红色假种皮中，紫杉醇 III 和 10-去乙酰基紫杉醇 III 的浓度最高。
结论
所开发的定量方法可靠且具有选择性，并成功应用于红豆杉浆果红色假种皮中选定的红豆杉成分的定量分析。证明了浆果的红色假种皮不含有选定的红豆杉化合物。
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