Department of Pediatrics, Xingtai People's Hospital, Xingtai, Hebei Province, China.
Eur Rev Med Pharmacol Sci. 2018 Jan;22(2):492-497. doi: 10.26355/eurrev_201801_14200.
To explore the changes of surfactant protein C, D (SP-C, SP-D) and apoptosis of alveolar epithelial cells (AEC) in the lung injury of neonatal rats induced by hyperoxia.
We divided neonatal rats within 24 hours into two groups randomly: the air group (n = 50) and the hyperoxia group (n = 50). Rats in the air group and hyperoxia group were bred conventionally and in tanks with normal pressure and 90% concentration of oxygen, respectively. On the 1st, 3rd, 7th, 10th, and 14th day after exposure, lung tissue of 8 rats in each group was collected and stained with hematoxylin and eosin (HE). We observed and recorded pathologic changes of lung tissue and detected apoptosis rate of alveolar epithelial cells by TUNEL (TdT-mediated dUTP nick end labeling). We detected the content of SP-C, SP-D in bronchoalveolar lavage fluid (BALF) by ELISA (enzyme-linked immunosorbent assay).
In the air group, the alveolar formed gradually with equable size and regular shape during growth. While the amount of alveolar decreased gradually and we observed small vessels dilation and increasing hemorrhage as well as increasing interstitial cells and swollen lung tissue in the hyperoxia group. In the air group, the content of SP-C in the BALF reduced during growth. However, the content of SP-C in the hyperoxia group was lower than that in the air group on the first day, higher on the third day, reached the peak on the seventh day and began to decrease on the tenth day and more obviously on the fourteenth day. The level of SP-D in the air group declined gradually with growing. On the first day, the content of SP-D in the hyperoxia group was similar to that in the air group. It began to increase on the third day, reached its peak on the seventh day, and began to decrease on the tenth day, more obviously on the fourteenth day.
Long-term exposure to hyperoxia inhibits the development of alveolar. The apoptosis of alveolar epithelial cells increased and the content of SP-C, SP-D in the lung tissue first increased and then decreased with the increase of exposure time.
探讨肺泡表面活性蛋白 C、D（SP-C、SP-D）和肺泡上皮细胞（AEC）凋亡在高氧诱导新生大鼠肺损伤中的变化。
将生后 24 小时内的新生大鼠随机分为两组：空气组（n=50）和高氧组（n=50）。空气组和高氧组大鼠分别在常压和 90%浓度的氧气罐中常规饲养。在暴露后的第 1、3、7、10 和 14 天，每组收集 8 只大鼠的肺组织，进行苏木精和伊红（HE）染色。观察和记录肺组织的病理变化，通过 TUNEL（末端转移酶介导的 dUTP 缺口末端标记）检测肺泡上皮细胞的凋亡率。通过 ELISA（酶联免疫吸附试验）检测支气管肺泡灌洗液（BALF）中 SP-C、SP-D 的含量。
空气组大鼠在生长过程中肺泡逐渐形成，大小均匀，形态规则。而高氧组大鼠肺泡数量逐渐减少，我们观察到小血管扩张和出血增加，间质细胞增多，肺组织肿胀。在空气组中，BALF 中的 SP-C 含量在生长过程中逐渐减少。然而，高氧组第 1 天的 SP-C 含量低于空气组，第 3 天升高，第 7 天达到高峰，第 10 天开始下降，第 14 天更为明显。空气组中 SP-D 的水平逐渐下降。第 1 天，高氧组 SP-D 的含量与空气组相似。第 3 天开始增加，第 7 天达到高峰，第 10 天开始下降，第 14 天更为明显。
长期暴露于高氧会抑制肺泡的发育。肺泡上皮细胞凋亡增加，肺组织中 SP-C、SP-D 的含量随暴露时间的增加先升高后降低。
