University Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria.
Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
Vet Microbiol. 2018 Feb;215:77-82. doi: 10.1016/j.vetmic.2018.01.006. Epub 2018 Jan 27.
The aim of this study was to determine the prevalence, the antimicrobial resistance patterns and the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian ruminants and New World camelids that were treated at the University of Veterinary Medicine, Vienna. Between April 2014 and January 2017, 723 nasal swabs originating from ruminants and New World camelids were examined. MRSA isolates were characterized by mecA/mecA1/mecC PCRs and by DNA microarray analysis. They were genotyped by spa typing, dru typing, MLST and MLVA. Glycopolymer fingerprinting by FTIR spectroscopy was also performed. Antimicrobial susceptibility testing was conducted by agar disk diffusion. Twelve MRSA isolates were mecA-positive, whereas three were mecC-positive. The MRSA isolates carried five different SCCmec elements, and belonged to three sequence types (ST45, ST130, ST398). The MRSA isolates displayed seven different resistance phenotypes. The present study describes for the first time mecC-carrying MRSA isolates originating from domesticated animals in Austria. More systematic studies are needed to unravel the role of ruminants and New World camelids as reservoirs for MRSA as a potential risk for zooanthropogenic transmission.
本研究旨在确定在维也纳兽医大学接受治疗的奥地利反刍动物和新世界驼类动物中耐甲氧西林金黄色葡萄球菌（MRSA）的流行率、抗生素耐药模式和遗传多样性。在 2014 年 4 月至 2017 年 1 月期间，共检测了 723 份源自反刍动物和新世界驼类动物的鼻腔拭子。通过 mecA/mecA1/mecC PCR 和 DNA 微阵列分析对 MRSA 分离株进行了特征分析。通过 spa 分型、dru 分型、MLST 和 MLVA 对其进行了基因分型。还通过傅里叶变换红外光谱（FTIR）光谱法进行了糖聚合物指纹图谱分析。通过琼脂扩散法进行了抗生素敏感性测试。12 株 MRSA 分离株 mecA 阳性，3 株 mecC 阳性。MRSA 分离株携带五种不同的 SCCmec 元件，属于三种序列类型（ST45、ST130、ST398）。MRSA 分离株表现出七种不同的耐药表型。本研究首次描述了源自奥地利家养动物的携带 mecC 的 MRSA 分离株。需要进行更多的系统研究，以阐明反刍动物和新世界驼类动物作为 MRSA 储主的作用，因为这可能是动物园人类传播的潜在风险。
