Firth Clair L, Käsbohrer Annemarie, Pless Peter, Koeberl-Jelovcan Sandra, Obritzhauser Walter
Unit of Veterinary Public Health and Epidemiology, Institute of Food Safety, Food Technology & Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, Austria.
Veterinary Directorate and Administration, Styrian Provincial Government, 8010 Graz, Austria.
Antibiotics (Basel). 2022 Jan 18;11(2):124. doi: 10.3390/antibiotics11020124.
The assumed link between high levels of antimicrobial use on farms and selection for antimicrobial-resistant (AMR) bacteria on that farm remains difficult to prove. In the pilot study presented here, we analysed total antimicrobial use on 50 dairy farms in Austria and also collected environmental samples to ascertain whether specific AMR bacteria were present. Antimicrobial use (AMU) analysis was based on electronic veterinary treatment records over a one-year period. Faecal samples for the assessment of extended-spectrum beta-lactamase (ESBL)-producing were collected from cowsheds, calf pens, and youngstock housing areas, as well as dust samples from barns, to isolate methicillin-resistant (MRSA). Bacteriological cultures were carried out on selective agar. Farms were split into groups of 25 of the highest antimicrobial users and 25 of the lowest users. Overall, samples from 13/50 (26.0%) farms were found to be positive for the presence of ESBL-producing . Of these, eight farms were in the low user group and five were in the high user group. Only one farm was confirmed to harbour MRSA. Statistical analyses demonstrated that there was no significant difference in this study population between high or low antimicrobial use with respect to the presence of ESBL-producing on farms ( = 0.33). In conclusion, the presence of specific AMR bacteria on farms in this study population was not found to have a statistically proven relationship with their level of antimicrobial use.
农场中大量使用抗菌药物与该农场中抗菌药物耐药（AMR）细菌的选择之间的假定联系仍难以证明。在此呈现的试点研究中，我们分析了奥地利50个奶牛场的抗菌药物总使用情况，并收集了环境样本以确定是否存在特定的AMR细菌。抗菌药物使用（AMU）分析基于一年期间的电子兽医治疗记录。从牛舍、犊牛栏和幼畜饲养区采集粪便样本以评估产超广谱β-内酰胺酶（ESBL）的情况，同时从畜棚采集灰尘样本以分离耐甲氧西林金黄色葡萄球菌（MRSA）。在选择性琼脂上进行细菌培养。农场被分为抗菌药物使用量最高的25个农场组和最低的25个农场组。总体而言，在50个农场中的13个（26.0%）农场的样本中发现产ESBL的情况呈阳性。其中，8个农场属于低使用量组，5个农场属于高使用量组。仅一个农场被确认藏有MRSA。统计分析表明，在本研究群体中，农场中产ESBL情况在高抗菌药物使用量和低抗菌药物使用量之间没有显著差异（P = 0.33）。总之，在本研究群体的农场中，未发现特定AMR细菌的存在与其抗菌药物使用水平之间存在经统计学证实的关系。
