商业益生菌的抗微生物药物耐药基因评估。
Evaluation of commercial probiotics for antimicrobial resistance genes.
机构信息
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington 99163, USA.
出版信息
Can Vet J. 2021 Apr;62(4):379-383.
Abstract
The objective of this study was to determine if transferable antimicrobial resistance (AMR) genes are present in commercial animal probiotics. DNA was extracted from 50 probiotics, tested for the presence of bacterial DNA, and analyzed by polymerase chain reaction (PCR) for the presence of 8 transferrable AMR genes, including tetracycline, erythromycin, aminoglycoside, sulfonamide, and trimethoprim. Samples that were positive by PCR were confirmed by genome sequencing. Forty-seven (94%) products contained bacterial DNA. Of these, 97% contained at least 1 AMR gene, and 82% contained 2 or more. These results indicate that further evaluation of the risk for transmission of these AMR genes may be warranted.
摘要
本研究旨在确定商业动物益生菌中是否存在可转移的抗菌药物耐药性（AMR）基因。从 50 种益生菌中提取 DNA，检测细菌 DNA 的存在，并通过聚合酶链反应（PCR）分析 8 种可转移 AMR 基因（包括四环素、红霉素、氨基糖苷类、磺胺类和甲氧苄啶）的存在情况。通过 PCR 检测呈阳性的样本通过基因组测序进行确认。47（94%）个产品含有细菌 DNA。其中，97%至少含有 1 种 AMR 基因，82%含有 2 种或更多种。这些结果表明，可能需要进一步评估这些 AMR 基因传播的风险。
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