Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University.
Microbes Environ. 2020;35(1). doi: 10.1264/jsme2.ME19060.
We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the "PS protocol"), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numbers and the frequency of phylogenetically novel isolates under aerobic, nitrate reduction, and fermentation conditions. The PS protocol is useful for isolating both aerobic and anaerobic microorganisms.
我们之前已经证明，通过将磷酸盐和琼脂分别进行高压灭菌（称为“PS 方案”），对琼脂培养基的制备进行简单的改进，可以减少活性氧的产生，从而提高好氧微生物的培养能力。在此，我们使用废水处理系统中的污泥作为微生物来源，研究了 PS 方案对厌氧微生物培养的影响。PS 方案的应用增加了好氧、硝酸盐还原和发酵条件下的菌落数量和系统发育新颖分离株的频率。PS 方案可用于分离好氧和厌氧微生物。
