Shao Huanhuan, Tu Yayi, Wang Yijing, Jiang Chunmiao, Ma Long, Hu Zhihong, Wang Jiangfan, Zeng Bin, He Bin
Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
College of Life Sciences, Sichuan Normal University, Chengdu 610101, China.
Microorganisms. 2019 Jul 30;7(8):225. doi: 10.3390/microorganisms7080225.
Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous fungi, little information is available about . We investigated the effect of two oxidant agents (menadione sodium bisulfite, MSB, and hydrogen peroxide, HO) on cellular growth and antioxidant enzyme induction in . Results indicated severe inhibition of biomass and conidia production when high concentration of oxidants was used. Transcriptomic analysis showed an up-regulated expression of genes involved in oxidoreduction, such as catalase, glutathione peroxidase, and superoxide dismutase. In addition, it was observed that oxidative stress stimuli enhanced the expression of Yap1 and Skn7 transcription factors. Further, metabolomic analysis showed that glutathione content was increased in the oxidative treatments when compared with the control. Moreover, the content of unsaturated fatty acid decreased with oxidative treatment accompanying with the down-regulated expression of genes involved in linoleic acid biosynthesis. This study provided a global transcriptome characterization of oxidative stress response in A. oryzae, and can offer multiple target genes for oxidative tolerance improvement via genetic engineering.
氧化应激反应可保护生物体免受活性氧（ROS）的有害影响，活性氧会损害细胞成分并导致细胞内稳态紊乱。尽管在酵母和其他丝状真菌中对防御性生化机制已进行了广泛研究，但关于[此处原文缺失相关内容]的信息却很少。我们研究了两种氧化剂（亚硫酸氢钠甲萘醌，MSB，和过氧化氢，H₂O₂）对[此处原文缺失相关内容]细胞生长和抗氧化酶诱导的影响。结果表明，当使用高浓度氧化剂时，生物量和分生孢子产量受到严重抑制。转录组分析显示，参与氧化还原的基因，如过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的表达上调。此外，观察到氧化应激刺激增强了Yap1和Skn7转录因子的表达。进一步的代谢组分析表明，与对照相比，氧化处理时谷胱甘肽含量增加。此外，氧化处理伴随着参与亚油酸生物合成的基因表达下调，不饱和脂肪酸含量降低。本研究提供了米曲霉氧化应激反应的全局转录组特征，并可为通过基因工程提高氧化耐受性提供多个靶基因。
