He Bin, Tu Yayi, Jiang Chunmiao, Zhang Zhe, Li Yongkai, Zeng Bin
Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
Microorganisms. 2019 Apr 10;7(4):103. doi: 10.3390/microorganisms7040103.
has been used for the production of traditional fermentation and has promising potential to produce primary and secondary metabolites. Due to the tough cell walls and high drug resistance of , functional genomic characterization studies are relatively limited. The exploitation of selection markers and genetic transformation methods are critical for improving fermentative strains. In this review, we describe the genome sequencing of various strains. Recently developed selection markers and transformation strategies are also described in detail, and the advantages and disadvantages of transformation methods are presented. Lastly, we introduce the recent progress on highlighted topics in functional genomics including conidiation, protein secretion and expression, and secondary metabolites, which will be beneficial for improving the application of to industrial production.
已被用于传统发酵生产，并且在产生初级和次级代谢产物方面具有广阔的潜力。由于[具体微生物名称]细胞壁坚韧且耐药性强，其功能基因组特征研究相对有限。选择标记和遗传转化方法的开发对于改良[具体微生物名称]发酵菌株至关重要。在本综述中，我们描述了各种[具体微生物名称]菌株的基因组测序情况。还详细介绍了最近开发的选择标记和转化策略，并阐述了转化方法的优缺点。最后，我们介绍了[具体微生物名称]功能基因组学中突出主题的最新进展，包括分生孢子形成、蛋白质分泌与表达以及次级代谢产物，这将有助于提高[具体微生物名称]在工业生产中的应用。
