Department of Life Sciences, National Central University, Jhongli, Taiwan.
Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Environ Toxicol. 2018 May;33(5):579-586. doi: 10.1002/tox.22546. Epub 2018 Feb 10.
Ametrym (AMT) is the most widely used herbicide and frequently detected in the aquatic environment. AMT also represent a potential health risk to aquatic organisms and animals, including humans. However, little data are available on their toxicity to zebrafish (Danio rerio). The aim of the present study was to evaluate the toxicological effects of AMT exposure on zebrafish embryos. In the acute toxicity test, 6 hpf embryos were exposed to various concentrations of AMT for 24 or 48 h. The results indicated that AMT induced malformation in larvae. To investigate the toxicological mechanism on the protein expression level. A proteomic approach was employed to investigate the proteome alterations of zebra fish embryos exposed to 20 mg/L AMT for 48 h. Among 2925 unique proteins identified, 298 differential proteins (> or <1.3-fold, P < 0.05) were detected in the treated embryos as compared to the corresponding proteins in the untreated embryos. Gene ontology analysis showed that these up-regulated proteins were most involved in glycolysis, lipid transport, protein polymerization, and nucleotide binding, and the down-regulated proteins were related to microtubule-based process, protein polymerization, oxygen transport. Moreover, KEGG pathway analysis indicated that tight junction, ribosome, and oxidative phosphorylation were inhibited in the treated embryos. These findings provide new insight into the mechanisms of toxicity induced by AMT.
氨氯吡啶酸（AMT）是一种被广泛使用的除草剂，经常在水生环境中被检测到。AMT 也对水生生物和动物（包括人类）构成潜在的健康风险。然而，关于其对斑马鱼（Danio rerio）的毒性的数据很少。本研究旨在评估 AMT 暴露对斑马鱼胚胎的毒理学影响。在急性毒性试验中，将 6 hpf 的胚胎暴露于不同浓度的 AMT 中 24 或 48 小时。结果表明 AMT 诱导幼虫畸形。为了研究在蛋白质表达水平上的毒性机制，采用蛋白质组学方法研究了暴露于 20mg/L AMT 48 小时的斑马鱼胚胎的蛋白质组改变。在鉴定的 2925 个独特蛋白质中，与未经处理的胚胎中的相应蛋白质相比，处理胚胎中检测到 298 个差异蛋白质（>或<1.3 倍，P<0.05）。GO 分析表明，这些上调的蛋白质主要参与糖酵解、脂质转运、蛋白质聚合和核苷酸结合，而下调的蛋白质与微管过程、蛋白质聚合、氧转运有关。此外，KEGG 途径分析表明，处理后的胚胎中紧密连接、核糖体和氧化磷酸化受到抑制。这些发现为 AMT 诱导的毒性机制提供了新的见解。
